AI Article Synopsis
- Ginseng is a valuable natural medicine, but its long cultivation time and quality control issues hinder its use, with limited research on how transplantation impacts metabolic processes, particularly ginsenoside production.
- The study utilized advanced techniques like Liquid chromatography tandem mass spectrometry and transcriptome analysis to examine how ginsenoside levels change throughout different growth stages in transplanted ginseng.
- Results showed that ginsenoside content significantly increased after 6-8 years post-transplantation, with lipid metabolism and carbohydrate pathways being critical to enhancing ginsenoside yield, offering insights for improving cultivation practices.
Article Abstract
Background: Ginseng ( Mayer) is an important natural medicine. However, a long culture period and challenging quality control requirements limit its further use. Although artificial cultivation can yield a sustainable medicinal supply, research on the association between the transplantation and chaining of metabolic networks, especially the regulation of ginsenoside biosynthetic pathways, is limited.
Methods: Herein, we performed Liquid chromatography tandem mass spectrometry based metabolomic measurements to evaluate ginsenoside accumulation and categorise differentially abundant metabolites (DAMs). Transcriptome measurements using an Illumina Platform were then conducted to probe the landscape of genetic alterations in ginseng at various ages in transplantation mode. Using pathway data and crosstalk DAMs obtained by MapMan, we constructed a metabolic profile of transplantation Ginseng.
Results: Accumulation of active ingredients was not obvious during the first 4 years (in the field), but following transplantation, the ginsenoside content increased significantly from 6-8 years (in the wild). Glycerolipid metabolism and Glycerophospholipid metabolism were the most significant metabolic pathways, as Lipids and lipid-like molecule affected the yield of ginsenosides. Starch and sucrose were the most active metabolic pathways during transplantation Ginseng growth.
Conclusion: This study expands our understanding of metabolic network features and the accumulation of specific compounds during different growth stages of this perennial herbaceous plant when growing in transplantation mode. The findings provide a basis for selecting the optimal transplanting time.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10785232 | PMC |
| http://dx.doi.org/10.1016/j.jgr.2023.09.003 | DOI Listing |
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