Laser speckle contrast imaging (LSCI) is applied in various biomedical applications for full-field characterization of blood flow and tissue perfusion. The accuracy of the contrast interpretation and its conversion to the blood flow index depends on specific parameters of the optical system and scattering media. One such parameter is the polarisation of detected light, which is often adjusted to minimize specular reflections and image artefacts. The polarisation's effect on the detected light scattering dynamics and, therefore, the accuracy of LSCI data interpretation requires more detailed investigation. In this study, we used LSCI and Dynamic Light Scattering Imaging to evaluate the effects of the detected light polarisation when imaging perfusion in the mouse cortex. We found that cross-polarisation results in a shorter decorrelation time constant, a higher coherence degree and stronger dynamic scattering compared to the parallel-polarisation or no-polariser configurations. These results support the cross-polarisation configuration as the most optimal for brain cortex imaging and suggest against direct or calibrated comparisons between the contrast recordings made with different polarisation configurations.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10783896 | PMC |
http://dx.doi.org/10.1364/BOE.507367 | DOI Listing |
Background: The early detection of neurologic damage at the microscopic level when the disease is subclinical would facilitate intervention preventing progression or potentially reversing the condition. The early determination of drug efficacy could shorten the length of drug studies, thereby reducing research costs. The eye is the only place in the body where an artery, vein, and nerve can be directly visualized The nerve fiber layer of the retina is an outgrowth of the brain.
View Article and Find Full Text PDFCurr Drug Deliv
January 2025
Department of Hepatobiliary Surgery, Ruian People's Hospital, the Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325200, China.
Background: Pancreatic cancer is a highly malignant tumor with a poor prognosis, and current treatment methods have limited effectiveness. Therefore, developing new and more effective therapeutic strategies is crucial. This study aims to establish pH-responsive silk fibroin (SF) nanoparticles encapsulating β-hydroxyisovalerylshikonin (SF@β-HIVS) to enhance the therapeutic effects against pancreatic cancer.
View Article and Find Full Text PDFMol Oncol
January 2025
Department of Precision Medicine in Medical, Surgical and Critical Care (Me.Pre.C.C.), University of Palermo, Italy.
Extracellular vesicle (EV) monitoring can complement clinical assessment of cancer response. In this study, patients with advanced non-small cell lung cancer (NSCLC) undergoing osimertinib, alectinib, pembrolizumab or platinum-based chemotherapy ± pembrolizumab were enrolled. EVs were characterized using Bradford assay to quantify the circulating cell-free EV protein content (cfEV), and dynamic light scattering to assess Rayleigh ratio excess at 90°, z-averaged hydrodynamic diameter and polydispersity index.
View Article and Find Full Text PDFBMC Cancer
January 2025
Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.
Background And Aim: Zinc oxide and copper oxide nanoparticles are known for their promising biological activities. This study aims to synthesize zinc oxide nanoparticles and copper-doped zinc oxide nanoparticles to harness the combined cytotoxic and anticancer effects of them in vitro and in vivo studies.
Methods: Zinc oxide nanoparticles, both doped and undoped, were synthesized using a chemical co-precipitation method.
Sci Rep
January 2025
ISQI, Faculty of Physics, Adam Mickiewicz University in Poznań, Poznań, Poland.
High-resolution Brillouin spectroscopy was employed to investigate the anisotropy in surface wave velocities within a bulk single crystal of SbTe, a well-known layered van der Waals material. By leveraging the bulk elastic constants derived from various simulation methods, we were able to theoretically calculate the distribution of surface acoustic phonon velocities on the cleavage plane of the material. Upon analyzing multiple simulation results, it became evident that the most significant discrepancies arose in the calculations of the elastic constant c, with values ranging from 48 to 98 GPa.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!