A taxonomic revision and a key to the pseudoscorpion species of Neobisium Chamberlin, 1930so far attributed to the subgenus Ommatoblothrus Beier, 1956from Corsica, Sardinia and mainland Italy are provided. The taxonomic status of the subgenera of Neobisium Chamberlin, 1930 is evaluated and the following new synonymies are proposed: Ommatoblothrus Beier, 1956 is a junior subjective synonym of Neobisium Chamberlin, 1930 (n. syn.), Heoblothrus Browning, 1965 is a junior subjective synonym of Neobisium Chamberlin, 1930 (n. syn.), Pennobisium uri 1988 is a junior subjective synonym of Neobisium Chamberlin, 1930 (n. syn.). The following new species are described: Neobisium (Neobisium) corsicum n. sp. (, loc. typ.: Corsica, Corte, Grotte de Valletto), Neobisium (N.) leolatellai n. sp. (, loc. typ.: Latium, Bassiano, Grotta della Cava 384 La/LT), Neobisium (N.) magrinii n. sp. (, loc. typ.: Latium, Coreno Ausonio, Grotta lArnale 871 La/FR), Neobisium (N.) petruzzielloi n. sp. (, loc. typ.: Campania, Avella, Grotta degli Sportiglioni 184 Cp/AV) and Neobisium (N.) vignai n. sp. (, loc. typ.: Latium, Amaseno, Grotta degli Ulivi 474 La/FR). The specimens of five populations from as many caves of the Lepini Mountains are provisionally attributed to Neobisium (N.) sp. aff. patrizii Beier, 1953, and the following new synonymy is proposed: Neobisium patrizii romanum Mahnert, 1980 is a junior subjective synonym of Neobisium patrizii patrizii Beier, 1953 (n. syn.).
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