A new species of the spider genus Ochyrocera Simon, 1891 is described from southern Mexico. Males and females of Ochyrocera marialuisae sp. nov. were collected from a cave in Oaxaca, Mexico. With the description of the new species herein, the species diversity of the genus from Mexico increases to eight species. In addition, biogeographical comments about the distribution of the species, an updated taxonomic identification key, and a distribution map for the Mexican species are provided.
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