Introducing fluorescent probe technology for detecting microcystin-LR in the water and cells.

Background: For a long time, the environment hazards caused by cyanobacteria bloom and associated microcystins have attracted attention worldwide. Microcystin-LR (MC-LR) is the most widely distributed and most toxic toxin. At present, numerous MC-LR detection methods exist many drawbacks. Therefore, a quick and accurate method for identifying and detecting MC-LR is crucial and necessary. In this work, we strived to introduce a novel fluorescence assay to detect MC-LR in the water and cells.

Results: According to the special spatial configuration and physicochemical properties of MC-LR, we designed and constructed six fluorescent probes. The design concepts of the probes were exhaustively elaborated. MC-YdTPA, MC-YdTPE, MC-RdTPA, and MC-RdTPE could show significant fluorescence enhancement in MC-LR solution. Significantly, MC-YdTPA, MC-YdTPE, and MC-RdTPA could also response well in the cells treated with MC-LR, demonstrating these fluorescent probes' values. The recognition mechanism between probes and MC-LR were also deeply explored: (1) The polyphenylene ring structure of probes may have nested or hydrogen bond weak interaction with the ring structure of MC-LR. (2) The probes can generate a reaction to the hydrogen ions ionized by MC-LR.

Significance: We proposed the novel ideas for designing MC-LR probes. This research can provide valuable experiences and important assistance in synthesizing MC-LR fluorescent probes. We expect that this work may bring new ideas to develop fluorescent probes for researching MC-LR in vivo and in vitro.