Early chronic kidney disease (CKD) has strong concealment and lacks an efficient, non-invasive, and lable-free detection platform. Cystatin C (Cys C) in urine is closely related to the progress of CKD (especially at the early stage), which is an ideal endogenous marker to evaluate the impairment of renal function. Thus, the accurate detection of urinary Cys C (u-Cys C) is great significant for early prevention and treatment and delaying the course of the disease of CKD patients. Herein, we developed an extended-gate field-effect transistor (EG-FET) sensor for ultrasensitive detection of u-Cys C, which consists of a monolithic interface-engineered graphene EG electrode array and a commercially available MOSFET. Laser-induced graphene (LIG) loaded with sputtered Au NPs in the presence of adhesive Cr (Au NPs/Cr/LIG) boosts the electrical performance of the EG electrode. Meanwhile, Au NPs also serve as linkers to immobilize papain that can selectively form protein complexes with Cys C. Supported by the synergistic effect of multilevel interface-engineered graphene, our sensor exhibits a good linear correlation within the u-Cys C concentration range of 5 ag/μL to 50 ng/μL with low detection limit of 0.05 ag/μL. Our work makes accurate, specific and rapid detection of u-Cys C feasible and promising for early screening for CKD.
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http://dx.doi.org/10.1016/j.bios.2024.116016 | DOI Listing |
Water Res
December 2024
Department of Civil and Environmental Engineering, Institute of Science Tokyo, 2-12-1, Meguro- Ku, Tokyo, 152-8552, Japan. Electronic address:
Intimately coupled photocatalytic biodegradation (ICPB) has been recently developed as an efficient wastewater treatment technique, particularly for removing persistent organic pollutants. However, photocatalyst/biofilm interaction in terms of photoelectron transfer and its effect on the overall performance of ICPB has not been explored. To investigate these points, interface-engineered composites of bismuth vanadate and reduced graphene oxide with low degree (BiVO/rGO-LC) and high degree of their contact (BiVO/rGO-HC) were fabricated and applied for ICPB.
View Article and Find Full Text PDFEnviron Res
September 2024
Department of Chemical and Biological Engineering, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam City, Gyeonggi-do, 461-701, South Korea. Electronic address:
A rational design of heterojunctions with high-quality contacts is essential for efficiently separating photogenerated charge carries and boosting the solar-driven harvesting capability. Herein, we fabricated a novel heterojunction of SnO quantum dots-anchored CdS-CdCO with g-CN nanosheets as a superior photocatalyst. SnO quantum dots (SQDs) with positively charged surfaces were tightly anchored on the negatively charged surface of CdS nanosheets (NSs).
View Article and Find Full Text PDFBiosens Bioelectron
April 2024
School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Dongchuan Road 500, Shanghai, 200241, China. Electronic address:
Early chronic kidney disease (CKD) has strong concealment and lacks an efficient, non-invasive, and lable-free detection platform. Cystatin C (Cys C) in urine is closely related to the progress of CKD (especially at the early stage), which is an ideal endogenous marker to evaluate the impairment of renal function. Thus, the accurate detection of urinary Cys C (u-Cys C) is great significant for early prevention and treatment and delaying the course of the disease of CKD patients.
View Article and Find Full Text PDFBiophys Rev (Melville)
December 2021
Preclinical education biochemistry, Lake Erie College of Osteopathic Medicine at Seton Hill, Greensburg, Pennsylvania 15601, USA.
The ability to manipulate the electrophysiology of electrically active cells and tissues has enabled a deeper understanding of healthy and diseased tissue states. This has primarily been achieved via input/output (I/O) bioelectronics that interface engineered materials with biological entities. Stable long-term application of conventional I/O bioelectronics advances as materials and processing techniques develop.
View Article and Find Full Text PDFJ Colloid Interface Sci
February 2022
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu, 225002, China; Key Laboratory of Organosilicon Chemistry and Material Technology of Ministry of Education, Hangzhou Normal University, Hangzhou 311121, People's Republic of China. Electronic address:
Conductive polymer nanofiber composites (CPNCs) based wearable sensing electronics have aroused great attention of scientists in recent years. However, it is still difficult to obtain CPNCs with good water proof, excellent durability, and multiple sensing performance. Herein, we develop a multifunctional CPNC with a wrinkled reduced graphene oxide (RGO) shell and polymer nanofiber core, which is prepared by ultrasonication induced decoration of RGO onto the pre-stretched polyurethane (PU) nanofibers, followed by the release of the strain.
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