A large number of bacterial small regulatory RNAs (sRNAs) modulate gene expression by base pairing to a target mRNA, affecting its translation or stability. This posttranscriptional regulation has been shown to be essential and critical for bacterial physiology. One of the challenges of studying sRNA signaling is identifying the sRNA regulators of specific genes. Here, we describe a protocol for making an sRNA expression library and using this library to screen for sRNA regulators of genes of interest in E. coli. This library can be easily expanded and adapted to use in other bacteria.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-3565-0_16 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
MicroRNAs in Plasma-Derived Extracellular Vesicles as Non-Invasive Biomarkers for Eosinophilic Esophagitis.
Int J Mol Sci
January 2025
Department of Gastroenterology, Hospital General de Tomelloso, 13700 Tomelloso, Spain.
Eosinophilic esophagitis (EoE) is a chronic inflammatory esophageal disorder. The lack of non-invasive biomarkers currently results in dependency on endoscopy with biopsies for its diagnosis and monitoring. We aimed to identify potential non-invasive biomarkers using microRNAs (miRNAs) in plasma-derived extracellular vesicles (pEVs).
View Article and Find Full Text PDFRNA Metabolism and the Role of Small RNAs in Regulating Multiple Aspects of RNA Metabolism.
Noncoding RNA
December 2024
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA.
RNA metabolism is focused on RNA molecules and encompasses all the crucial processes an RNA molecule may or will undergo throughout its life cycle. It is an essential cellular process that allows all cells to function effectively. The transcriptomic landscape of a cell is shaped by the processes such as RNA biosynthesis, maturation (RNA processing, folding, and modification), intra- and inter-cellular transport, transcriptional and post-transcriptional regulation, modification, catabolic decay, and retrograde signaling, all of which are interconnected and are essential for cellular RNA homeostasis.
View Article and Find Full Text PDFsRNA113 regulates motility to affect immune response against infection in pearl gentian grouper.
Zool Res
January 2025
Fisheries College, Jimei University, Xiamen, Fujian 361021, China. E-mail:
Small RNAs (sRNAs) are a class of molecules capable of perceiving environmental changes and exerting post-transcriptional regulation over target gene expression, thereby influencing bacterial virulence and host immune responses. is a pathogenic bacterium that poses a significant threat to aquatic animal health. However, the regulatory mechanisms of sRNAs in .
View Article and Find Full Text PDFComprehensive analysis of small RNA, transcriptome, and degradome sequencing: Mapping the miRNA-gene regulatory network for the development of sweet potato tuber roots.
Plant Physiol Biochem
January 2025
Guangxi Key Laboratory of Agro-environment and Agro-products Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, 530004, China; Guangxi University Key Laboratory of Crop Cultivation and Tillage, Nanning, 530004, China. Electronic address:
As an important starch crop, sweet potato has significant practical importance for maintaining food security worldwide. This study identified differential expressed genes associated with the expansion of tuberous roots by comparing the transcriptome across tuberous roots at the initial period (initiated tuberous roots (ITRs), rapid expansion period (tuberous roots (TRs), fibrous roots (FRs) at the seedling stage, and fibrous roots at the adult stage (unexpanded FRs (UFRs)). sRNA-seq and degradome analyses were performed to reveal the role of miRNAs in tuberous root development in sweet potato.
View Article and Find Full Text PDFRegulatory role of PA3299.1 small RNA in Pseudomonas aeruginosa biofilm formation via modulation of algU and mucA expression.
Biochem Biophys Res Commun
January 2025
Department of Microbiology, University of Kalyani, Kalyani, 741235, India. Electronic address:
Small RNAs (sRNAs) have emerged as key regulators of transcriptional factors and components within regulatory networks that govern bacterial biofilm formation. This study aimed to explore the regulatory role of the PA3299.1 sRNA in controlling biofilm formation in P.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!