Effects of Outer Membrane Vesicles on Intestinal Microbiota and Intestinal Barrier Function.

is one of the most important zoonotic pathogens causing foodborne gastroenteritis worldwide. Outer membrane vesicles (OMVs) are lipid-bilayer vesicles produced by Gram-negative bacteria, which contain biologically active components. We hypothesized that OMVs are an important weapon of to initiate enteric diseases pathologies. In this study, the effects of OMVs (OMVs) on intestinal microbiota and intestinal barrier function were investigated. fecal culture experiments showed that alpha diversity indexes and microbiota composition were altered by OMV supplementation. OMV supplementation showed an increase of pH, a decrease of OD630 and total short chain fatty acid (SCFA) concentrations. IPEC-J2 cells culture experiments showed that OMV supplementation did not affect the IPEC-J2 cell viability and the indicated genes expression. experiments in mice showed that OMVs had adverse effects on average daily gain ( < 0.05) and feed:gain ratio ( < 0.05), and had a tendency to decrease the final body weight ( = 0.073) in mice. OMV administration decreased serum interleukin-10 level ( < 0.05), decreased the relative abundance of bacteria belonging to the genera -018 and ( < 0.05). Furthermore, OMV administration damaged the ileum mucosa ( < 0.05). These findings suggest that OMVs play an important role in the activation of intestinal inflammatory response induced by , and downregulation of SCFA-producing bacteria is a possible mechanism.