Two novel strains were isolated from wetland soils in Goyang, Republic of Korea. The two Gram-stain-positive, facultatively anaerobic, rod-shaped bacterial-type strains were designated MW4 and MW9. Phylogenomic analysis based on whole-genome sequences suggested that both strains belonged to the genus . The cells of strain MW4 were non-motile and grew at 20-40 °C (optimum, 35 °C), at pH 6.0-10.0 (optimum, pH 8.0) and in the presence of 0-1.0% NaCl (optimum, 0 %). The cells of strain MW9 were non-motile and grew at 20-40 °C (optimum, 35 °C), at pH 5.0-9.0 (optimum, pH 8.0) and in the presence of 0-1.0% NaCl (optimum, 0 %). The average nucleotide identity (77.1-88.1 %) and digital DNA-DNA hybridization values (21.0-34.8 %) between the two novel strains and with their closely related strains fell within the range for the genus . The novel strains MW4 and MW9 and reference strains possessed alkane synthesis gene clusters (, , and ). Phylogenomic, phylogenetic, average nucleotide identity, digital DNA-DNA hybridization, physiological and biochemical data indicated that the novel strains were distinct from other members of the family . We propose the names sp. nov. (type strain MW4=KACC 23260=TBRC 17645) and sp. nov. (type strain MW9=KACC 23261=TBRC 17646) for the two strains.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1099/ijsem.0.006235 | DOI Listing |
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!