Differentially expressed of RNA-seq data are suggested on the intersections of normalization techniques.

Biochem Biophys Rep

Clinical Biochemistry Department, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.

Published: March 2024

Data normalization is the critical step for the RNA-seq data analysis. Several techniques are suggested for the normalization of transcript reads in the samples. In this study, the differentially expressed (DEGs) are generated from the TCGA normalized laryngeal cancer data obtained using the TPM, FPKM, and DESeq2 techniques. The results showed that the reports of DEGs were different based on the normalization techniques. We suggested that the DEG intersections obtain the top transcripts from the normalized data in support of the pathway enrichment process.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10776424PMC
http://dx.doi.org/10.1016/j.bbrep.2023.101618DOI Listing

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