AI Article Synopsis
- The study addresses the public health issue of rising carbapenemase-producing infections, specifically focusing on New Delhi metallo-β-lactamase-1 (NDM-1) producing strains.
- Researchers isolated a carbapenem-resistant strain from a patient's stool sample and analyzed its phenotypic and genomic characteristics, confirming the presence of the resistance gene on a conjugative plasmid.
- The findings highlight the need for ongoing surveillance to effectively control and prevent the spread of these dangerous infections.
Article Abstract
Purpose: The continuous rise in carbapenemase-producing infections is a major public health concern. However, there is limited information available on New Delhi metallo-β-lactamase-1 (NDM-1) producing . In this study, we isolated a -carrying from a stool sample of an inpatient. Our aim was to investigate the phenotypic and genomic features of this clinically derived carbapenem-resistant isolate and to characterize the transmission pattern of the IncFII/IncN plasmid that carries the gene.
Methods And Results: S1-PFGE, Southern blot and conjugation assay confirmed the presence of gene in a conjugative plasmid. L2395 and transconjugant L2395-EC600 strains showed similar resistance spectrum. Whole-genome analysis revealed that pL2395_NDM is an IncFII/IncN plasmid with a length of 67,839 bp. Moreover, gene was found encoded in the IS--IS cassette array. Phylogenetic analysis revealed that strain L2395 was close to an IMP-4-bearing from Australia.
Conclusion: Ongoing surveillance will be essential to control and prevent the spread of carbapenem-resistant spp. in the future.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10778185 | PMC |
| http://dx.doi.org/10.2147/IDR.S435771 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of an NDM-1-Producing Isolate from China.
Infect Drug Resist
January 2024
Department of Critical Care Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.
Article Synopsis
- The study addresses the public health issue of rising carbapenemase-producing infections, specifically focusing on New Delhi metallo-β-lactamase-1 (NDM-1) producing strains.
- Researchers isolated a carbapenem-resistant strain from a patient's stool sample and analyzed its phenotypic and genomic characteristics, confirming the presence of the resistance gene on a conjugative plasmid.
- The findings highlight the need for ongoing surveillance to effectively control and prevent the spread of these dangerous infections.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!