The Targeted Regulation of BDUbc and BDSKL1 Enhances Resistance to Blight in × .

is the major pathogen responsible for the significant stem disease "blight" in × . The interacting proteins of the key pathogenic factor , BDUbc and BDSKL1, have previously been obtained by two-hybrid, BiFC, GST pull-down yeast assays. However, the functions of these interacting proteins remain unknown. This study successfully obtained transgenic plants overexpressing , , and + via Agrobacterium-mediated gene overexpression. qRT-PCR analysis revealed significantly increased expression levels of and in the transgenic plants. After infection with the pathogenic spore suspension, the disease incidence and severity index significantly decreased across all three transgenic plants, accompanied by a marked increase in defense enzyme levels. Notably, the co-transformed plant, OE- + , demonstrated the lowest disease incidence and severity index among the transgenic variants. These results not only indicate that and are disease-resistant genes, but also that these two genes may exhibit a synergistic enhancement effect, which further improves the resistance to blight in × .