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Matrine Targets BTF3 to Inhibit the Growth of Canine Mammary Tumor Cells. | LitMetric

Matrine Targets BTF3 to Inhibit the Growth of Canine Mammary Tumor Cells.

Int J Mol Sci

Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China.

Published: December 2023

AI Article Synopsis

  • - The study explores the use of a canine mammary tumor model to investigate human breast cancer, focusing on the effects of matrine and its biotin-labeled probe on canine mammary epithelial cells and specific tumor cell lines (CHMm and CHMp).
  • - Safe concentrations of matrine were found to be 250 μg/mL and 500 μg/mL for the probe, with both showing a time-dependent inhibition of cell growth and the induction of autophagy.
  • - Further analysis revealed that matrine specifically targets the BTF3 protein, decreasing its expression and stability over time, as confirmed by qPCR and Western blot experiments.

Article Abstract

The canine mammary tumor model is more suitable for studying human breast cancer, and the safety concentrations of matrine and the biotin-labeled matrine probe were determined in canine primary mammary epithelial cells, and then selected canine mammary tumor cell lines CHMm and CHMp were incubated with matrine, and cell viability was detected by CCK-8. The biotin-labeled matrine probe was used to pull-down the targets of matrine in canine mammary tumor cells, and the targets were screened in combination with activity-based protein profiling (ABPP) and Genecards database, and verified by qPCR and western blot. The results showed that the maximum non-cytotoxic concentrations of matrine and biotin-labeled matrine probe in canine primary mammary epithelial cells were 250 μg/mL and 500 μg/mL, respectively. Matrine and biotin-labeled matrine probe had a proliferation inhibitory effect time-dependently on CHMm and CHMp cells within a safe concentration range, and induced autophagy in cells. Then BTF3 targets were obtained by applying ABPP and Genecards screening. Cellular thermal shift assay (CETSA) findings indicated that matrine could increase the heat stability of BTF3 protein. Pull-down employing biotin-labeled matrine probe with CHMm and CHMp cell lysates revealed that BTF3 protein was detected in the biotin-labeled matrine probe group and that BTF3 protein was significantly decreased by the addition of matrine. The qPCR and western blot findings of CHMm and CHMp cells treated with matrine revealed that matrine decreased the expression of the BTF3 gene and protein with the extension of the action time, and the impact was more substantial at the protein level, respectively.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10779273PMC
http://dx.doi.org/10.3390/ijms25010540DOI Listing

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