WOX family transcription factors are well-known regulators of plant development, controlling cell proliferation and differentiation in diverse organs and tissues. Several genes have been shown to participate in regeneration processes which take place in plant cell cultures in vitro, but the effects of most of them on tissue culture development have not been discovered yet. In this study, we evaluated the effects of gene overexpression on the embryogenic callus development and transcriptomic state in . According to our results, overexpression of leads to an increase in callus weight. Furthermore, transcriptomic changes in overexpressing calli are, to a large extent, opposite to the changes caused by overexpression of , a somatic embryogenesis stimulator. These results add new information about the mechanisms of interaction between different genes and can be useful for the search of new regeneration regulators.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10780917 | PMC |
http://dx.doi.org/10.3390/plants13010102 | DOI Listing |
BMC Plant Biol
December 2024
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Background: Embryogenic callus (EC) has strong regenerative potential, useful for propagation and genetic transformation. miRNAs have been confirmed to play key regulatory roles in EC regeneration across various plants. However, challenges in EC induction have hindered the breeding of drumstick (Moringa oleifera Lam.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
School of Agriculture, Yunnan University, Kunming 650500, China.
() genes play significant roles in plant development and stress responses. Difficulties in somatic embryogenesis are a significant constraint on the uniform seedling production and genetic modification of , hindering efforts to improve coffee production in Yunnan, China. This study comprehensively analyzed genes in three species.
View Article and Find Full Text PDFPLoS One
December 2024
Agricultural Research Station, Fort Valley State University, Fort Valley, Georgia, United States of America.
An efficient regeneration system was established through somatic embryogenesis and shoot organogenesis using mature embryos explants of peanut cultivar 'Georgia-12Y'. The role of plant growth regulator combinations was investigated for embryogenic callus and somatic embryo induction. Results showed that Murashige and Skoog (MS) medium supplemented with 20 μM picloram (4-amino 3, 5, 6-trichloropicolinic acid), casein hydrolysate (0.
View Article and Find Full Text PDFPlants (Basel)
November 2024
Istituto di Bioscienze e BioRisorse (IBBR), Consiglio Nazionale delle Recerche, Via Ugo La Malfa 153, 90146 Palermo, Italy.
Somatic embryogenesis (SE) provides alternative methodologies for the propagation of grapevine ( spp.) cultivars, conservation of their germplasm resources, and crop improvement. In this review, the current state of knowledge regarding grapevine SE as applied to these technologies is presented, with a focus on the benefits, challenges, and limitations of this method.
View Article and Find Full Text PDFFor Res (Fayettev)
October 2024
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Here, a DNA-free genetic editing approach is presented for larch by delivering ribonucleoprotein complexes (RNPs) of CRISPR/Cas9 through particle bombardment. The detailed procedure encompasses creating a transgenic system particle bombardment for the transformation of embryogenic callus, validating the functionality of RNPs, optimizing coating and delivery techniques, enhancing somatic embryo maturation, regenerating plantlets, and precisely identifying mutants. The optimal particle bombardment parameters were determined at 1,100 psi and a distance of 9 cm and the editing efficiency of the targets was verified .
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!