The objective of the present study was to determine the effects that the reproductive season has on the motility, kinematics, morphology, and sperm morphometry of Brahman bulls evaluated with a commercial CASA system. The experiment was carried out at the Costa Rica Institute of Technology from March to August 2021. A total of eight Brahman bulls were used. A total of 28 ejaculates were collected in the pre-mating period (PMP), during it (DMP), and after it (AMP) using an electroejaculator. The sperm concentration was measured with the Accuread photometer. The motility was measured using a Spermtrack counting chamber. The analyses were performed with the CASA-Mot ISASv1 system. The morphology was analyzed using a microscope with a negative phase contrast objective. Morphometry was evaluated with the CASA-Morph. The sperm concentration did not present differences between the PMP and AMP; however, it was significantly higher than DMP ( > 0.05). Regarding the progressiveness variables, linearity on forward progression (LIN), straightness (STR), and wobble (WOB) were higher ( < 0.05) DMP. A kinematic principal component analysis grouped all the variables into three factors and an effect on the reproductive period was found ( < 0.05) in the parameters of the head and middle part of the sperm, such as width and perimeter, which were greater in the PMP. The length of the sperm head in the PMP and DMP did not show differences; however, both were larger ( < 0.05) than AMP. The insertion distance of the middle piece of the sperm was significantly greater than DMP. Finally, the PMP contained cells with a larger insertion angle ( < 0.05) than AMP. These findings are important to understand the implications of reproductive status on sperm quality and to consider them in andrological evaluations.
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http://dx.doi.org/10.3390/ani14010132 | DOI Listing |
PLoS One
November 2024
Department of Animal and Dairy Science, University of Georgia, Athens, Georgia, United States of America.
The Sardo Negro cattle (SN) is the only zebu cattle breed developed in Mexico. Since its development, the selection could have led to an increase in the homozygosity level in some regions of the genome and made differentiation with other cattle populations. We aimed to identify and characterize selection signatures in SN using medium-density SNP data using four approaches: 1) Runs of homozygosity (ROH) 2) Nucleotide Diversity 3) Tajima's D and 4) the Wright's fixation index (FST).
View Article and Find Full Text PDFTrop Anim Health Prod
October 2024
Departamento de Zootecnia, Facultad de Ciencias Agropecuarias, Grupo de Investigación en Reproducción y Mejoramiento Genético Animal, Universidad de Sucre, Sincelejo, Sucre, Colombia.
Supplementation with insulin-like growth factor type 1 (IGF-1) during in vitro culture of bovine embryos has yielded mixed results, likely due to genetic variability among embryos. This work aimed to evaluate the effect of IGF-1 at two concentrations on the development speed embryos from primary F1 crossbreeds used in dual-purpose cattle farming in the Colombian low tropics. Specifically, we investigated the influence of IGF-1 and embryo breed on the blastocyst formation rate.
View Article and Find Full Text PDFJ Parasitol Res
October 2024
Center for Teaching, Research, and Extension in Tropical Livestock, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Mexico City 93600, Mexico.
Grazing management through pasture rotation has been mentioned as a viable alternative for the control of ; however, there is limited information on rotational grazing at field level. The objectives of this research were (1) to evaluate the effect of rotational grazing with 30 and 45 days of pasture rest and continuous grazing (without rest) on tick loads in Brahman heifers and its most common crosses with Holstein; (2) to know the parasite dynamics of under the three grazing systems in the humid tropics of Mexico; and (3) to determine the effect of the treatments on the characteristics of the pastures (availability of forage biomass, plant height, and soil cover). The experiment was carried out for 1 year from April 2022 to March 2023, with three grazing treatments: Treatments 1 and 2 considered rotational grazing with 30 (RT30) and 45 days of rest (RT45), respectively, and Treatment 3 as continuous grazing (CT00).
View Article and Find Full Text PDFGigascience
January 2024
The Davies Research Centre, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy SA 5371, Australia.
Background: Most DNA methylation studies have used a single reference genome with little attention paid to the bias introduced due to the reference chosen. Reference genome artifacts and genetic variation, including single nucleotide polymorphisms (SNPs) and structural variants (SVs), can lead to differences in methylation sites (CpGs) between individuals of the same species. We analyzed whole-genome bisulfite sequencing data from the fetal liver of Angus (Bos taurus taurus), Brahman (Bos taurus indicus), and reciprocally crossed samples.
View Article and Find Full Text PDFTrop Anim Health Prod
September 2024
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
The bovine leukocyte antigen (BoLA) gene is a significant genetic part of the immune system and has been used as a disease marker in cattle. In this study, we detected Theileria orientalis, T. sinensis, Anaplasma marginale, Anaplasma platys, Candidatus Mycoplasma haemobos and Trypanosoma evansi by PCR amplification and sequencing of the amplicons.
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