sp. nov., a heavy-metal-tolerant bacterium isolated from garden soil.

A previously undescribed, heavy-metal-tolerant, motile, Gram-negative bacterium, designated strain SK50-23, was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SK50-23 was closely related to LMG 26467 and the non-phototrophic '' NS23 (98.1 and 97.3 % 16S rRNA gene sequence similarity, respectively). Strain SK50-23 possessed a circular genome of 5.86 Mb, with a DNA G+C content of 61.9 mol%. Digital DNA-DNA hybridization showed 20.8-21.6 % similarity between strain SK50-23 and related species. In addition, the whole-genome average nucleotide identity values between strain SK50-23 and related species ranged from 75.1 to 83.5 %. The major cellular fatty acid identified in strain SK50-23 was Cω7, and the main isoprenoid quinone present was ubiquinone Q-10. Strain SK50-23 could be assigned to the genus with the species name sp. nov. based on morphological, chemotaxonomic and genome-based taxonomic characteristics, and 16S rRNA gene-based phylogenetic characteristics. The type strain of the proposed novel species is SK50-23 (=NBRC 108825=CGMCC No. 1.12037).