Occurrence, genetic diversity and resistance profiles of from Brazilian sausages collected at production facilities.

Unlabelled: This study aimed to investigate the occurrence and the genetic diversity of subsp. in sausages from Southern Brazil, evaluate virulence genes and determine the phenotypic and genotypic basis of antimicrobial and sanitizer resistance. was detected in sausage samples with an overall prevalence of 5.5%. The prevalent serovars were . Infantis and . Rissen. Pulsed-field gel electrophoresis (PFGE) analysis yielded nine distinct PFGE profiles, and some of them were recurrently recovered in the same establishment on different dates. Among tested isolates, 28.5% showed resistance to at least one antimicrobial agent and a multidrug-resistance (MDR) profile was observed in 21.4%. Resistance occurred most frequently to ampicillin, sulfonamide, trimethoprim/sulfamethoxazole, and trimethoprim. Regarding the genotypic antimicrobial resistance profile, Schwarzengrund carried (B), , , and genes. Benzalkonium chloride and chlorhexidine were more effective than peracetic acid and sodium hypochlorite, showing lower minimum inhibitory concentration values. Six serovars were found, demonstrating a potential risk of salmonellosis associated with consuming this food. carrying virulence genes, MDR profile, and tolerance to sanitizers is a public health concern and a challenge for the food industry, suggesting that new strategies should be developed to control this pathogen.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-023-05809-w.