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Two pairs of back-to-back α-helices of Kingella kingae RtxA toxin are crucial for the formation of a membrane pore.
Int J Biol Macromol
December 2024
Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic. Electronic address:
The RtxA cytotoxin, a member of the RTX (Repeats in ToXin) family of pore-forming toxins, is the primary virulence factor of the paediatric facultative pathogen Kingella kingae. Although structure-function studies of RTX toxins have defined their characteristic domains and features, the exact membrane topology of RTX toxins remains unknown. Here, we used labelling of cell-bound RtxA with a membrane-impermeable, lysine-reactive reagent and subsequent detection of the labelled lysine residues by mass spectrometry, which revealed that most of the membrane-bound toxin is localised extracellularly.
View Article and Find Full Text PDFClinical Utility of a Multiplex PCR Panel (BioFire Joint Infection) in the Adjustment of Empiric Antimicrobial Therapy: Experience in Pediatric Osteoarticular Infections.
Children (Basel)
October 2024
CIBERINFEC, Consorcio de Investigación Biomédica en Red, Hospital Carlos III, 28029 Madrid, Spain.
Background/objectives: This study aims to evaluate the impact of the PCR multiplex panel (BioFire JI) on the diagnosis and management of pediatric osteoarticular infections.
Methods: This retrospective study analyzed data from pediatric patients diagnosed with osteoarticular infections between January 2023 and April 2024. The effectiveness of the PCR multiplex panel in identifying pathogens was compared with traditional culture methods.
Correlation between the results of cultures and the molecular BIOFIRE® joint infection panel in a cohort of pediatric patients with bone and joint infections in Bogotá, Colombia.
Front Pediatr
April 2024
Departamento de Microbiología, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.
Article Synopsis
- Bone and Joint Infections (BJI) have significant health impacts, particularly with the rising prevalence of Methicillin-resistant Staphylococcus aureus (MRSA), making accurate and timely diagnosis crucial.
- A study analyzed data from pediatric patients suspected of having BJI, comparing traditional culture methods with the Biofire joint infection panel (BJIP) to evaluate their correlation in diagnosis.
- The findings indicated a moderate overall agreement between the two diagnostic methods, with the BJIP proving effective in identifying fastidious and polymicrobial bacteria, while achieving perfect concordance in detecting MRSA.
and Coinfection of Brain: An Unusual Case from China.
Infect Drug Resist
April 2024
Department of Clinical laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
Background: The HACEK group comprises spp., , , , and , are Gram-negative bacteria that are slow-growing and fastidious. These organisms are common causes of culture-negative endocarditis.
View Article and Find Full Text PDFPotential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study.
J Bone Jt Infect
February 2024
bioMérieux, Marcy l'Etoile, France.
Article Synopsis
- The BIOFIRE Joint Infection (JI) Panel uses multiplex-PCR testing to identify microorganisms in synovial fluid from patients suspected of septic arthritis and prosthetic joint infections.
- A study over 34 clinical sites in Europe and the Middle East indicated that the JI Panel showed 88.4% agreement in diagnosing septic arthritis and 85% for prosthetic joint infections when compared to traditional synovial fluid cultures.
- The JI Panel not only detected more positive samples, especially anaerobic bacteria, but also provided results in about one hour, improving patient management and treatment decisions.
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