The negative impact of fish processing industry waste needs to be minimized, by processing it into valuable products, one of which is fish feed. The objectives of this research were to determine the optimum dose of crude extract of RGL.1.1 enzyme in hydrolyzing protein from the waste of catfish () fillet processing and to evaluate the effectiveness of using protein hydrolysate in snakehead fish () feed. There were four doses of enzyme treatment for protein hydrolysis designed in a completely randomized design, namely, 0, 4, 6, and 8% (v/w) with three repetitions. Furthermore, to assess the quality of protein hydrolysate, an analysis of soluble protein level, hydrolysis degree, amino acid content, fatty acid content, and digestibility was carried out. The percentage of protein hydrolysate applied in the feed formula was 0, 15, 30, and 45% (v/w), which was designed in a completely randomized design with three replications. Absolute weight growth, specific growth rate, protein efficiency ratio, feed efficiency, and snakehead fish survival were measured to evaluate the effects of the feed formula. Results showed that the crude extract of RGL.1.1 enzymes at a concentration of 6% (v/w) enhanced the availability of soluble proteins, amino acids, fatty acids, and feed digestibility. Protein hydrolysate application in snakehead fish feed formula up to 45% (v/w) can improve the growth performance (8.03%), protein efficiency ratio (25.66%), and feed efficiency (23.41%).
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http://dx.doi.org/10.1155/2023/2815122 | DOI Listing |
Food Res Int
January 2025
Department of Food Science, Université Laval, Québec G1V 0A6, Canada; Laboratoire de Transformation Alimentaire et Procédés ÉlectroMembranaires (LTAPEM, Laboratory of Food Processing and ElectroMembrane Processes), Université Laval, Québec G1V 0A6, Canada; Institute of Nutrition and Functional Foods (INAF), Université Laval, Québec G1V 0A6, Canada. Electronic address:
Industrial wastewaters are significant global concerns due to their environmental impact. Yet, protein-rich wastewaters can be valorized by enzymatic hydrolysis to release bioactive peptides. However, achieving selective molecular differentiation and eventually enhancing peptide bioactivities require costly cascades of membranes.
View Article and Find Full Text PDFFood Res Int
January 2025
State Key Laboratory of Food Science and Resources, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Guangdong Engineering Research Center of High-Value Utilization and Equipment Development of Marine Biological Resources, Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, Guangdong 511458, China; Jiangnan University (Shaoxing) Industrial Technology Research Institute, Shaoxing, Zhejiang 31200, China; National Engineering Research Center of Huangjiu, Zhejiang Guyuelongshan Shaoxing Wine CO., LTD, Shaoxing 646000, Zhejiang, China. Electronic address:
To explore the mechanism of Virgibacillus proteases on hydrolysis of shrimp myofibrillar protein (SMP) and formation of volatile compounds, the fermented broth of Virgibacillus halodenitrificans was purified and the protease was identified as peptidase S8. The enzyme had optimum activity at pH 7.0-8.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Center for Mitochondria and Healthy Aging, School of Life Sciences, Yantai University, Yantai 264005, China; College of Life Sciences, Yantai University, Yantai 264005, Shandong, China. Electronic address:
Erectile dysfunction (ED) is a male sexual disorder mainly caused by a reduction in the cellular concentration of cyclic guanosine monophosphate (cGMP), which is degraded by phosphodiesterase type-5 (PDE-5). Oyster protein (OP) and its hydrolysates have been used for centuries to address male erectile dysfunction, however the mechanisms and evidence supporting their efficacy remain unclear. In this study, OP was hydrolyzed using trypsin to produce peptides that inhibit PDE-5.
View Article and Find Full Text PDFAnim Cells Syst (Seoul)
December 2024
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
Insect protein hydrolysates (PH) are emerging as valuable compounds with biological activity. The aim of the present study was to assess the potential cytoprotective effects of PH from the Black Soldier Fly (BPH, in the range 0.1-0.
View Article and Find Full Text PDFNutrients
December 2024
School of Sport, Exercise & Nutrition, College of Health, Massey University, Palmerston North 4410, New Zealand.
Background: Consuming collagen hydrolysate (CH) may improve symptoms of exercise-induced muscle damage (EIMD); however, its acute effects have not been compared to dairy protein (DP), the most commonly consumed form of protein supplement. Therefore, this study compared the effects of CH and DP on recovery from EIMD.
Methods: Thirty-three males consumed either CH ( = 11) or DP ( = 11), containing 25 g of protein, or an isoenergetic placebo ( = 11) immediately post-exercise and once daily for three days.
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