[Emerging and comparative genomic analysis of a novel plasmid carrying in ].

To explore the drug resistance mechanism and gene structure characteristics of a carbapenemase-producing novel incompatibility group plasmid pNY2385-KPC from . A multi-drug resistant strain was obtained from urine samples of patients with fever in the emergency ward of Li Huili Hospital, Ningbo Medical Center. Bacterial species was preliminary identified and finally confirmed by 16S rRNA gene amplification and the average nucleotide identity alignment, respectively. The minimum inhibitory concentrations of the antimicrobial agents were determined by VITEK 2 Compact System. The complete genome sequence was obtained by "third-generation" sequencing methods, and then detailed annotation of gene function and comparative genomic analysis of plasmid structure were carried out by BLASTP/BLASTN, RefSeq, ConservedDomains, ResFinder, Isfinder, etc. The pNY2385-KPC carried by NY2385 belonged a novel incompatibility group, and contained and conjugative transfer (type Ⅳ secretory system, T4SS) genes, which could induce conjugative transfer. A total of 15 plasmids of the same type as pNY2385-KPC were retrieved by NCBI, which were from , and the rest were from , , , , and other bacteria, and were broad-host-range plasmids. The sequence comparative analysis of all 6 of the novel plasmid from showed that the structure of the novel plasmid had certain conserved property, with Tn variant structure carrying , and plasmid pCF1807-3 had both and . The pNY2385-KPC type plasmids in carried resistance gene, which were divided into two subtypes: single replicator and / complex replicator, belonging to broad-host-range plasmids. And as a mobile genetic element, the plasmids promote the spread of .