AI Article Synopsis
- Fibre typing through immunohistochemistry on frozen human muscle biopsies is crucial for diagnosing and researching muscle-related diseases and effects of exercise.
- Proper preservation of these cryosections can be difficult when stored for extended periods, especially in large studies.
- This study shows that the morphology and reactivity of important muscle markers remain intact even after 18 years of storage, allowing for reliable image analysis related to muscle fiber composition and capillarization.
Article Abstract
Fibre typing by immunohistochemistry on cryosections from human skeletal muscle biopsies is an essential tool in the diagnosis and research of muscular diseases, ageing, and responses to exercise training and disuse. Preserving a good quality in these frozen specimens can be challenging especially if they are stored for longer periods before histological processing, which is often the case in studies with a large number of test subjects and/or repeated sampling separated by multiple years. We demonstrate in this article that both, the morphology and reactivity of epitopes to myosin heavy chain isoforms and dystrophin are well preserved in up to 18-year-stored unfixed and unstained cryosections of human m. vastus lateralis (n = 241). Any variation in staining intensity between samples was unrelated to the age of the biopsy donor or the storage period of the unstained cryosections, and in all cases, the obtained images were appropriate for image analysis, such as the determination of the fibre type composition and the fibre cross-sectional area, and quantitative analysis of muscle capillarisation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11021626 | PMC |
| http://dx.doi.org/10.1111/joa.14003 | DOI Listing |
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