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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10788607 | PMC |
| http://dx.doi.org/10.3348/kjr.2023.0948 | DOI Listing |
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4D live tracing reveals distinct movement trajectories of meiotic chromosomes.
Life Med
December 2024
Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
Proper chromosome alignment at the spindle equator is a prerequisite for accurate chromosome segregation during cell division. However, the chromosome movement trajectories prior to alignment remain elusive. Here, we established a 4D imaging analysis framework to visualize chromosome dynamics and develop a deep-learning model for chromosome movement trajectory classification.
View Article and Find Full Text PDFAutomated Laser-Assisted Single-Cell Sorting for Cell Functional and RNA Sequencing.
ACS Sens
January 2025
Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, P. R. China.
Accurate and efficient sorting of single target cells is crucial for downstream single-cell analysis, such as RNA sequencing, to uncover cellular heterogeneity and functional characteristics. However, conventional single-cell sorting techniques, such as manual micromanipulation or fluorescence-activated cell sorting, do not match current demands and are limited by low throughput, low sorting efficiency and precision, or limited cell viability. Here, we report an automated, highly efficient single-cell sorter, integrating laser-induced forward transfer (LIFT) with a high-throughput picoliter micropore array.
View Article and Find Full Text PDFDeciphering key nano-bio interface descriptors to predict nanoparticle-induced lung fibrosis.
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State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Suzhou Medical School, Soochow University, Suzhou, Jiangsu, 215123, China.
Background: The advancement of nanotechnology underscores the imperative need for establishing in silico predictive models to assess safety, particularly in the context of chronic respiratory afflictions such as lung fibrosis, a pathogenic transformation that is irreversible. While the compilation of predictive descriptors is pivotal for in silico model development, key features specifically tailored for predicting lung fibrosis remain elusive. This study aimed to uncover the essential predictive descriptors governing nanoparticle-induced pulmonary fibrosis.
View Article and Find Full Text PDFMacro-Micro Properties of Remodeled Waste Slurry Under Freeze-Thaw Cycles.
Materials (Basel)
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School of Civil and Transportation Engineering, Hebei University of Technology, Xiping Road 5340, Tianjin 300401, China.
Waste slurry, a major by-product of urban construction, is produced in rapidly increasing volumes each year. Dehydrated waste slurry has potential as a roadbed material; however, its performance in freeze-thaw environments, which can induce frost heave and thaw settlement, and the mechanism of the influence of freeze-thaw cycles on its macro and micro properties are still unclear and need thorough investigation. This study explores the macroscopic and microscopic properties of waste slurry subjected to freeze-thaw cycles.
View Article and Find Full Text PDFEffects of Far-Red Light and Ultraviolet Light-A on Growth, Photosynthesis, Transcriptome, and Metabolome of Mint ( Briq.).
Plants (Basel)
December 2024
College of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
To investigate the effects of different light qualities on the growth, photosynthesis, transcriptome, and metabolome of mint, three treatments were designed: (1) 7R3B (70% red light and 30% blue light, CK); (2) 7R3B+ far-red light (FR); (3) 7R3B+ ultraviolet light A (UVA). The results showed that supplemental FR significantly promoted the growth and photosynthesis of mint, as evidenced by the increase in plant height, plant width, biomass, effective quantum yield of PSII photochemistry (F'/F'), maximal quantum yield of PSII (F/F), and performance index (PI). UVA and CK exhibited minimal differences.
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