The objective of this study was to characterize ICEChn2, a novel SXT/R391-related integration and conjugation element (ICE) carrying 19 drug resistance genes, in a clinical isolate of from swine. Whole genome sequencing (WGS) of CP063424 strain was completed using a combination of third-generation PacBio and second-generation Illumina. The putative ICE was predicted by the online tool ICEfinder. ICEChn2 was analyzed by PCR, conjugation experiments, and bioinformatics tools. CP063424 strain exhibited high minimum inhibitory concentrations of clindamycin (1,024 mg/L). The WGS data revealed that ICEChn2, with a length of 167,870 bp and encoding 151 genes, including multiple antibiotic resistance genes such as , was found to be related to the SXT/R391 family on the chromosome of CP063424. The circular intermediate of ICEChn2 was detected by PCR, but conjugation experiments showed that it was not self-transmissible. To our knowledge, ICEChn2 is the longest member with the most resistance genes in the SXT/R391 family. Meanwhile, ATP-binding cassette superfamily was found to be inserted in the ICEChn2 and possessed a new insertion region, which is the first description in the SXT/R391 family.
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