Moonlighting proteins, known for their ability to perform multiple, often unrelated functions within a single polypeptide chain, challenge the traditional "one gene, one protein, one function" paradigm. As organisms evolved, their genomes remained relatively stable in size, but the introduction of post-translational modifications and sub-strategies like protein promiscuity and intrinsic disorder enabled multifunctionality. Enzymes, in particular, exemplify this phenomenon, engaging in unrelated processes alongside their primary catalytic roles. This study employs a systematic, quantitative informatics approach to shed light on human moonlighting protein sequences. Phylogenetic analyses of human moonlighting proteins are presented, elucidating the distal-proximal relationships among these proteins based on sequence-derived quantitative features. The findings unveil the captivating world of human moonlighting proteins, urging further investigations in the emerging field of moonlighting proteomics, with the potential for significant contributions to our understanding of multifunctional proteins and their roles in diverse cellular processes and diseases.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2023.128998 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nucleus-translocated GCLM promotes chemoresistance in colorectal cancer through a moonlighting function.
Nat Commun
January 2025
Department of Medical Oncology, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, PR China.
Metabolic enzymes perform moonlighting functions during tumor progression, including the modulation of chemoresistance. However, the underlying mechanisms of these functions remain elusive. Here, utilizing a metabolic clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 knockout library screen, we observe that the loss of glutamate-cysteine ligase modifier subunit (GCLM), a rate-limiting enzyme in glutathione biosynthesis, noticeably increases the sensitivity of colorectal cancer (CRC) cells to platinum-based chemotherapy.
View Article and Find Full Text PDFPolarised moonlight guides nocturnal bull ants home.
Elife
December 2024
School of Natural Sciences, Macquarie University, Sydney, Australia.
For the first time in any animal, we show that nocturnal bull ants use the exceedingly dim polarisation pattern produced by the moon for overnight navigation. The sun or moon can provide directional information via their position; however, they can often be obstructed by clouds, canopy, or the horizon. Despite being hidden, these bodies can still provide compass information through the polarised light pattern they produce/reflect.
View Article and Find Full Text PDFExtracellular nicotinamide phosphoribosyltransferase visfatin activates JAK2-STAT3 pathway in cancer-associated fibroblasts to promote colorectal cancer metastasis.
Genes Genomics
December 2024
School of Bioscience and Technology, Chengdu Medical College, Chengdu, China.
Article Synopsis
- Metastasis poses a significant challenge in treating colorectal cancer (CRC), with cancer-associated fibroblasts (CAFs) playing a crucial role in this process.
- The study investigates the effects of visfatin, an extracellular protein, on CAFs and how it influences CRC metastasis through mechanisms involving inflammatory factors and the JAK-STAT signaling pathway.
- Results show that visfatin enhances the invasive and migratory abilities of CRC cells by activating inflammatory responses in CAFs, suggesting that targeting this interaction could be a potential therapeutic strategy for CRC.
Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles.
Mol Cell
December 2024
MRC, University of Glasgow Centre for Virus Research, Glasgow G61 1QH, Scotland, UK. Electronic address:
RNA is a central molecule for viruses; however, the interactions that viral RNA (vRNA) establishes with the host cell is only starting to be elucidated. Here, we determine the ribonucleoprotein (RNP) composition of the prototypical arthropod-borne Sindbis virus (SINV). We show that SINV RNAs engage with hundreds of cellular proteins, including a group of nuclear RNA-binding proteins (RBPs) with unknown roles in infection.
View Article and Find Full Text PDFProtein-Variant-Phenotype Study of NBAS Using AlphaFold in the Aspect of SOPH Syndrome.
Proteins
December 2024
Research Laboratory of "Molecular Medicine and Human Genetics", Institute of Medicine, Ammosov North-Eastern Federal University, Yakutsk, Republic of Sakha (Yakutia), Russia.
NBAS gene variants cause phenotypically distinct and nonoverlapping conditions, SOPH syndrome and ILFS2. NBAS is a so-called "moonlighting" protein responsible for retrograde membrane trafficking and nonsense-mediated decay. However, its three-dimensional model and the nature of its possible interactions with other proteins have remained elusive.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!