AI Article Synopsis
- The study focuses on improving cryopreservation techniques for ovarian tissue by examining the influence of factors like corpus luteum presence and the side of the ovaries on cell viability and morphology.
- The researchers categorized bovine ovaries into six groups based on whether they had a corpus luteum and their side (right or left), finding that ovaries without a corpus luteum and from the right side showed the best tissue preservation.
- Findings indicate that recognizing the origin of the ovarian samples can enhance the success of ovarian tissue vitrification, suggesting a new approach for better outcomes in cryopreservation.
Article Abstract
Although cryopreservation of ovarian tissue has advanced greatly, it remains a challenge, and protocols should be optimized to handle the heterogeneous nature of ovarian samples. In an effort to address this factor, the present study evaluated the effects of corpus luteum (CL) and side of ovaries (right versus left) on cellular morphology and viability of vitrified bovine ovarian fragments in a closed system. The ovaries were categorized according to whether they had a CL and which side they were on, and then divided into six groups: 1) CL+ (with CL) group; 2) CL- (without CL) group; 3) right ovaries group; 4) left ovaries group; 5) fresh control group (ovaries without vitrification or culture that were not selected for CL or ovarian side) and 6) culture medium control group (non-vitrified ovaries that were not selected for the presence or absence of CL or side of the ovaries). The current study shows that the CL- and right groups had the greatest percentage of follicles with normal morphology compared to other vitrified-warmed groups. Furthermore, the levels of necrosis and tissue damage of the right cultured group were the lowest compared to other groups. It was shown that bovine ovarian tissues derived from right ovaries and ovaries without a corpus luteum can be functionally and morphologically preserved after vitrification. For the first time, the present study suggests that bovine ovarian tissue vitrification can be improved by considering the origin of the ovaries.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759775 | PMC |
| http://dx.doi.org/10.30466/vrf.2023.1972637.3684 | DOI Listing |
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