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| http://dx.doi.org/10.3389/fgene.2023.1348372 | DOI Listing |
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Systemic evaluation of various CRISPR/Cas13 orthologs for knockdown of targeted transcripts in plants.
Genome Biol
December 2024
Hubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
Article Synopsis
- The CRISPR/Cas13 system, known for its compactness and specificity in targeting RNA, shows potential for knocking down endogenous transcripts in plants, but its subtypes need more exploration.* -
- This research evaluates seven Cas13 orthologs from five subtypes, finding that RfxCas13d, Cas13x.1, and Cas13x.2 demonstrated high editing efficiencies (58-80%) and the ability to simultaneously target two RNA transcripts.* -
- The findings suggest a new method for transcriptome editing in plants, which could enhance crop improvement efforts and provide insights for functional research.*
Next-generation marker-free transplastomic plants: engineering the chloroplast genome without integration of marker genes in Solanum tuberosum (potato).
Plant Cell Rep
November 2024
Center for Agricultural Synthetic Biology (CASB), University of Tennessee, 2640 Morgan Circle Dr., Knoxville, TN, 37996, USA.
This study describes an optimized plastid genetic engineering platform to produce full marker-free transplastomic plants with transgene integrated at homoplasmy in one step in tissue culture. Plastid engineering is attractive for both biotechnology and crop improvement due to natural bio-confinement from maternal inheritance, the absence of transgene positional effects and silencing, the ability to express transgenes in operons, and unparalleled production of heterologous proteins. While plastid engineering has had numerous successes in the production of high-value compounds, no transplastomic plants have been approved for use in agriculture.
View Article and Find Full Text PDFThe actions of varenicline on alkaloids from Conium maculatum (poison hemlock), Lupinus sulphureus (sulphur lupine) and Nicotiana glauca (tree tobacco).
Toxicon
December 2024
United States Department of Agriculture, Agricultural Research Service, Poisonous Plant Research Laboratory, 1150 East 1400 North, Logan, UT, 84341, USA.
Evidence-based therapies to manage the clinical signs of intoxication caused by toxic plants in livestock are lacking. For that reason, the aim of this work was to develop a drug-based intervention for the management of clinical signs of piperidine alkaloid intoxication in livestock. The actions of anabasine, coniine, γ-coniceine, and two total alkaloid extracts from Lupinus sulphureus were compared in the presence and absence of the nicotinic acetylcholine receptor partial agonist varenicline in RD cells, mice and goats.
View Article and Find Full Text PDFChemical composition and antifungal activity of pepper aqueous extracts against plant pathogens and food spoilage fungi.
Front Cell Infect Microbiol
October 2024
Department of Chemical Science and Natural Resources, Universidad de La Frontera, Temuco, Chile.
Article Synopsis
- Pepper is loaded with beneficial phytochemicals like capsaicinoids and phenols, which have antimicrobial and antioxidant properties useful for both human and plant health.
- In a study, different pepper varieties (Cacho de Cabra, Bell Pepper, Hungarian Wax, and Cristal) were assessed for their capsaicin and phenolic content, finding that Cristal and Hungarian Wax had the highest capsaicinoids while Bell Pepper had the most phenols and antioxidant activity.
- The research demonstrated that extracts from these peppers can inhibit fungal growth, showing their potential as non-toxic pest management tools in agriculture.
Genomic prediction for potato (Solanum tuberosum) quality traits improved through image analysis.
Plant Genome
December 2024
Department of Horticultural Science, University of Minnesota, Saint Paul, Minnesota, USA.
Potato (Solanum tuberosum L.) is the most widely grown vegetable in the world. Consumers and processors evaluate potatoes based on quality traits such as shape and skin color, making these traits important targets for breeders.
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