In DAF-7/TGF-beta signaling regulates development, metabolism, and behavior. In addition loss of leads to an increase of the glutamate receptor GLR-1. In mutants, GLR-1 tagged with GFP (GLR-1::GFP) accumulates in wide puncta along the ventral nerve cord of the animal. Previous automated analyses of GLR-1::GFP accumulation relied on the proprietary software, IgorPro, for measurement of GLR-1::GFP puncta size, intensity, and density. We did a side-by-side comparison of analyses by IgorPro and an open source macro written for Fiji to analyze images from animals expressing GLR-1::GFP in wild type and backgrounds. Analyses by the two programs were in strong agreement and are in accordance with previously published data on the effects of on GLR-1::GFP accumulation. Based on these data, we conclude that the Fiji platform is a robust method for analyzing the accumulation of a fluorescently-tagged neurotransmitter receptor and that the Fiji puncta plugin will be applicable for image analysis for other neural markers.
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| http://dx.doi.org/10.17912/micropub.biology.001004 | DOI Listing |
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