Detection and Genotyping of Samples in Species of Genus from Different Environments in the Brazilian Amazon.

In the Amazon region, several species of triatomines occur in the natural environments. Among them, species of the genus are a risk to human populations due to their high rates of infection with The aim of this study was to identify the genotypes in specimens and their relationship with sylvatic hosts from different environments in the Brazilian Amazon. A total of 492 triatomines were collected from the municipalities of Monte Negro, Rondônia state, and Humaitá, Amazonas state, 382 of them being nymphs and 110 adults. Genotyping of in six discrete typing units (DTUs) was performed using conventional multilocus PCR. The triatomines that were positive for and engorged with blood were also targeted for amplification of the gene to identify bloodmeal sources. Of the 162 positive samples, the identified DTUs were TcI (87.65%) and TcIV (12.35%). It was observed that 102 specimens were engorged with a variety of bloodmeals. Triatomines infected with TcI were associated with DNA of all identified vertebrates, except TcIV was detected in triatomines that fed on , , , , , , and Results highlight the need to understand the patterns of genotypes in spp. and their association with sylvatic hosts to better elucidate their role in the transmission of Chagas disease in the Amazon region.