AI Article Synopsis
- - This study aimed to identify and analyze the bacteria causing pneumonic pasteurellosis in Bonga sheep from Southwest Ethiopia, focusing on their antibiotic resistance and impact on sheep productivity.
- - Researchers used cultured nasal swabs from sheep at veterinary clinics, applying microbiological tests and species-specific PCR methods to confirm the presence of the bacteria and assess their antibiotic susceptibility.
- - The results showed that the isolation rate of pathogens was around 31.76%, with notable resistance to common antibiotics like chloramphenicol and penicillin G, confirming specific bacterial species as the causative agents of the disease.
Article Abstract
Background: Pneumonic pasteurellosis is a respiratory system disease of sheep caused by , and responsible for the low productivity and economic loss resulting from death and treatment costs. This study was conducted to isolate and molecularly detect causative agents and antibiotic susceptibility tests from a nasal swab sample of the Bonga sheep breed that was suspected to have pneumonic pasteurellosis in selected areas of Southwest Ethiopian Peoples Regional State.
Methods: A cross-sectional study design was used along with purposive sampling of nasal swab samples from sheep that were brought to veterinary clinics during the study period. Bacterial isolation and phenotypic characterization were carried out using microbiological and biochemical tests that followed standard microbiological techniques. To molecularly confirm the isolates, and , species-specific PCR primer genes were used. Using the disc diffusion method, molecularly confirmed isolates were subjected to an in vitro antibiotic susceptibility test.
Results: The 85 samples that were scrutinized had an overall isolation rate of 31.76%, whereas the isolates of and had species compositions of 40.7% and 59.25%, respectively. Overall, 12.5% of the and 18.18% of the species were verified from phenotypical isolates using the species-specific PCR primer genes and , respectively. An in vitro antibiotic susceptibility test was carried out on all four PCR-confirmed isolates for seven commonly used antibiotics used to treat ovine pasteurellosis in the study area. It was found that both bacterial species were resistant to chloramphenicol and penicillin G.
Conclusion: Using phenotypic and molecular diagnostic techniques, the results of our current inquiry revealed that and are the causative agents of ovine pneumonic pasteurellosis in the study area.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10757770 | PMC |
| http://dx.doi.org/10.2147/VMRR.S435932 | DOI Listing |
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