Objective: To uncover and identify the differences in salivary microbiota profiles and their potential roles between patients with pulmonary nodules (PN) and healthy controls, and to propose new candidate biomarkers for the early warning of PN.
Methods: 16S rRNA amplicon sequencing was performed with the saliva samples of 173 PN patients, or the PN group, and 40 health controls, or the HC group, to compare the characteristics, including diversity, community composition, differential species, and functional changes of salivary microbiota in the two groups. Random forest algorithm was used to identify salivary microbial markers of PN and their predictive value for PN was assessed by area under the curve (). Finally, the biological functions and potential mechanisms of differentially-expressed genes in saliva samples were preliminarily investigated on the basis of predictive functional profiling of Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2).
Results: The α diversity and β diversity of salivary microbiota in the PN group were higher than those in the HC group (<0.05). Furthermore, there were significant differences in the community composition and the abundance of oral microorganisms between the PN and the HC groups (<0.05). Random forest algorithm was applied to identify differential microbial species. , , and constituted the optimal marker sets (=0.79, 95% confidence interval: 0.71-0.86), which can be used to effectively identify patients with PN. Bioinformatics analysis of the differentially-expressed genes revealed that patients with PN showed significant enrichment in protein/molecular functions involved in immune deficiency and redox homeostasis.
Conclusion: Changes in salivary microbiota are closely associated with PN and may induce the development of PN or malignant transformation of PN, which indicates the potential of salivary microbiota to be used as a new non-invasive humoral marker for the early diagnosis of PN.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10752765 | PMC |
| http://dx.doi.org/10.12182/20231160103 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Impact of Electronic and Conventional Cigarette Use towards Saliva Profile and Oral Microbiota in Adolescents.
Asian Pac J Cancer Prev
January 2025
Department of Rural Clinical Science, La Trobe Rural Health School, La Trobe University, Melbourne, Australia.
Objective: To compare the salivary profiles of smokers (e-cigarette smokers, e-cigarette and former conventional cigarette smokers, dual users, and conventional cigarette smokers) and non-smokers in adolescents, focusing on acidity level, flow rate, viscosity, as well as the quantity of Streptococcus mutans, Porphyromonas gingivalis, and Candida albicans.
Methods: This analytical observational study, with a cross-sectional design, involves collecting saliva samples from five groups through the draining method. Saliva viscosity was assessed visually, while saliva flow rate was monitored over a ten-minute period.
Systematic analyses uncover robust salivary microbial signatures and host-microbiome perturbations in oral squamous cell carcinoma.
mSystems
January 2025
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, China.
Unlabelled: Oral squamous cell carcinoma (OSCC) is a prevalent malignancy in the oral-maxillofacial region with a poor prognosis. Oral microbiomes play a potential role in the pathogenesis of this disease. However, findings from individual studies have been inconsistent, and a comprehensive understanding of OSCC-associated microbiome dysbiosis remains elusive.
View Article and Find Full Text PDFDistinctive salivary oral microbiome in patients with burning mouth syndrome depending on pain intensity compared to healthy subjects.
J Dent Sci
January 2025
Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan, Republic of Korea.
Background/purpose: Burning moouth syndrome (BMS) is a chronic pain condition similar to neuropathic pain. It is characterized by a persistent burning sensation in the oral cavity. Despite the lack of clarity regarding the etiology of BMS, recent studies have reported an association between the gut microbiome and neuropathic pain.
View Article and Find Full Text PDFRelationship Between the Salivary Microbiome and Oral Malodor Metabolites in Older Thai Individuals with Periodontitis and the Cytotoxic Effects of Malodor Compounds on Human Oral Squamous Carcinoma (HSC-4) Cells.
Dent J (Basel)
January 2025
Institute of Dentistry, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
: Halitosis is primarily caused by the activity of oral microorganisms. In this study, we employed metagenomic sequencing and metabolomic approaches to investigate the differences in salivary microbiota and metabolite profiles between individuals with halitosis and periodontitis and healthy controls. Additionally, we expanded the study to examine how oral malodorous compounds interact with human oral squamous carcinoma (HSC-4) cells.
View Article and Find Full Text PDFThe interplay of edentulism, smoking, microbiota, oral rehabilitation on cytokineprofile and different conditions of hypertension.
Arch Oral Biol
January 2025
Department of Dental Materials and Prosthesis, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address:
Objective: To evaluate the influence of edentulism, smoking, microbiota, and oral rehabilitation on the cytokine profile in healthy and hypertensive edentulous individuals using complete dentures.
Design: This case-control study was divided into four groups: normotensives (control group - NH), controlled hypertensives (case group 1 - CH), unreported hypertensives (case group 2 - UnrH), and uncontrolled hypertensives (case group 3 - UncH). The participants were characterized by sociodemographic data, clinical and behavioral information, and systolic and diastolic blood pressure.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!