Biotoxicity attenuation and the underlying physicochemical mechanism of biochar aged under simulated natural environmental conditions.

Biochar (BC), with the benefits of enhancing soil fertility, absorbing heavy metals, carbon sequestration, and mitigating the greenhouse effect, has been extensively used for soil remediation. However, the long-term changes in the biotoxicity of BC under complex environmental conditions, which are the key factors influencing the sustainable application of BC in soil, are still unclear. Herein, the biotoxicity of BC aged with various processes, including dry‒wet cycle (DW) aging, freeze‒thaw cycle (FT) aging, ultraviolet irradiation (UV) aging, and low molecular weight organic acid (OA) aging, was systematically investigated by Escherichia coli (E. coli) culture experiments. The toxicity attenuation rate (%·week) was proposed to more concisely and clearly compare the influence of different aging methods on BC toxicity. The results indicated that after 5 weeks of aging, the toxicity attenuation rate during the four aging modes followed the order OA aging > FT aging > UV aging > DW aging. BC was nontoxic after 1 week of OA aging, 4 weeks of FT aging, 7 weeks of UV aging, and 14 weeks of DW aging. Spectroscopic characterizations revealed that humic acids in the dissolved organic matter of BC were the main reason for the biotoxicity. In addition, the attenuation of environmentally persistent free radicals on BC during aging was also an important factor for reducing environmental toxicity. This work provides insight into the detoxification mechanism of the BC aging process under ordinary environmental conditions and guidance for the safe application of BC in soil.