Effect of on adipocyte differentiation in goats.

Cholesterol is regarded as a signaling molecule in regulating the metabolism and function of fat cells, in which 7-Dehydrocholesterol reductase (DHCR7) is a key enzyme that catalyzes the conversion of 7-dehydrocholesterol to cholesterol, however, the exact function of in goat adipocytes remains unknown. Here, the effect of on the formation of subcutaneous and intramuscular fat in goats was investigated , and the result indicated that the mRNA level of showed a gradual downward trend in subcutaneous adipogenesis, but an opposite trend in intramuscular adipogenesis. In the process of subcutaneous preadipocytes differentiation, overexpression of inhibited the expression of adipocytes differentiation marker genes (, , and ), lipid metabolism-related genes (, , and ), and the lipid accumulation. However, in intramuscular preadipocyte differentiation, overexpression showed a promoting effect on adipocyte differentiation marker genes (, , and ) and lipid metabolism-related genes (, , and ) expression, and on lipid accumulation. In summary, our work demonstrated that played an important role in regulating adipogenic differentiation and lipid metabolism in preadipocytes in goats, which is of great significance for uncovering the underlying molecular mechanism of adipocyte differentiation and improving goat meat quality.