AI Article Synopsis
- Human umbilical cord blood (UCB) mononuclear cells show promise for treating ischemic stroke (IS), but the specific role of SSEA3 positive subpopulations was previously unexplored.
- In this study, researchers isolated SSEA3(+)UCB cells, which made up about 7.01% of UCB cells, and found that these cells exhibited distinctive markers and higher Oct3/4 expression.
- After injecting SSEA3(+)UCB cells into a rat model of ischemic stroke, the treated group showed better neurological recovery and less neuronal loss compared to the control group, suggesting that SSEA3 positive cells may be effective in IS therapy.
Article Abstract
Numerous evidences showed that human umbilical cord blood (UCB) mononuclear cells were a promising approach for the therapy of ischemic stroke(IS). The effect of stage-specific embryonic antigen 3 (SSEA3）positive subpopulation in UCB was not investigated in IS. In this study, we isolated SSEA3 positive cells from healthy UCB mononuclear cells, which comprised about 7.01% of the total UCB-mononuclear cells. Flow cytometry analysis revealed that SSEA3(+)UCB cells were almost positive for CD44 and CD45, and negative for CD73, CD90 and CD105. The expression of Oct3/4 in SSEA3(+)UCB cells were higher than that in UCB. SSEA3(+)UCB cells sorted by magnetic cell sorting were intravenously injected into the middle cerebral arterial occlusion(MCAO) rat model. Neurological score showed that SSEA3(+)UCB transplantation group exhibited significant improvements in the functional outcome of MCAO rats than UCB transplantation group. Nissl staining and microtubule association protein-2(MAP2) immunofluorescence staining showed that the SSEA3(+)UCB transplantation group decreased neuronal loss. SSEA3(+)UCB transplantation group reduced neuronal apoptosis, inhibited caspase3 expression, and decreased tumor necrosis factor α(TNF-α). These results indicate that SSEA3 positive cells are a novel subpopulation of UCB cells, which exhibit great potential for the treatment of ischemic stroke.
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| http://dx.doi.org/10.1016/j.neures.2023.12.006 | DOI Listing |
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