Objectives: To characterize an Escherichia coli strain causing bloodstream infection encoding both high-virulence and carbapenem-resistance phenotypes.
Methods: Antimicrobial susceptibility testing, WGS and bioinformatics analysis were performed to characterize strain E1. The function of the ColV plasmid was investigated by the Galleria mellonella infection model, serum killing and macrophage killing assays. The fitness effect of the ColV plasmid was tested by growth curve, plasmid stability tests and the in vitro competition assay. The conjugation assay was performed to test the transferability of the ColV and blaNDM-5-carrying plasmids.
Results: E. coli E1 from bloodstream infection was MDR and highly virulent in the G. mellonella infection model. It belonged to phylogroup D, ST38 and serotype O7:H8. E1 carried a conjugatively transferable IncI1-type blaNDM-5-positive plasmid, which conferred carbapenem resistance, a conjugative IncFIB/FII-type ColV plasmid encoding an array of virulence-associated genes and antibiotic resistance genes blaTEM-1B, strAB and sul2, and seven other plasmids. Co-transfer of the ColV plasmid and the blaNDM-5-positive plasmid was observed. The ColV virulence-resistance hybrid plasmid contributed to the virulence, resistance to serum killing, and macrophage phagocytosis in E. coli E1. The carriage of this ColV plasmid did not constitute an in vitro fitness burden to strain E1 but caused fitness costs to E. coli strain EC600.
Conclusions: The emergence of such a highly virulent and resistant strain with conjugative blaNDM-5-positive and ColV plasmids posed a significant threat to public health. Implementation of control measures is needed to prevent such strains from further disseminating in hospital settings and the community.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/jac/dkad403 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Major F plasmid clusters are linked with ColV and pUTI89-like marker genes in bloodstream isolates of Escherichia coli.
BMC Genomics
January 2025
Australian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW, Australia.
Background: F plasmids are abundant in E. coli, carrying a variety of genetic cargo involved in fitness, pathogenicity, and antimicrobial resistance. ColV and pUTI89-like plasmids have drawn attention for their potential roles in various forms of extra-intestinal pathogenicity.
View Article and Find Full Text PDFDietary zinc supplementation inhibits bacterial plasmid conjugation by regulating plasmid replication () and transfer () genes.
Appl Environ Microbiol
October 2024
Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, Iowa, USA.
Article Synopsis
- Humans use dietary supplements to address malnutrition, but their impact on gut microbiota is not well understood, particularly regarding antibiotic resistance genes.
- This study investigates how dietary zinc supplements affect the transfer of multidrug resistance plasmids between bacteria, using specific bacterial strains and standardized assays.
- Results show that zinc supplementation significantly reduces bacterial populations involved in plasmid transfer and suggests that zinc could be a promising strategy to combat antibiotic resistance in both humans and animals.*
Occurrence and genomic characterization of antimicrobial-resistant and potential pathogenic from Italian artisanal food productions of animal origin.
Ital J Food Saf
May 2024
Department of Agricultural and Food Sciences, Alma Mater Studiorum University of Bologna, Ozzano dell'Emilia.
can harbor a broad repertoire of virulence and antimicrobial resistance (AMR) genes, which can be exchanged across the human gastrointestinal microflora, thus posing a public health risk. In this study, 6 batches of artisanal soft cheese and a 6-month ripened fermented dried sausage were investigated to assess the occurrence, phylogeny, and genomic traits (AMR, virulence, and mobilome) of . 30 and 3 strains isolated from salami and cheese food chains, respectively, were confirmed as by whole genome sequencing.
View Article and Find Full Text PDFHorizontal transfer characterization of ColV plasmids in bla-bearing avian Escherichia coli.
Poult Sci
May 2024
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, PR China; Key Laboratory of Quality and Safety Control of Poultry Products (Zhengzhou), Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, PR China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou 450046, PR China; Zhengzhou Key Laboratory of Research and Evaluation of Traditional Chinese Veterinary Medicine, Zhengzhou 450046, PR China. Electronic address:
Extended-spectrum-β-lactamases (ESBLs)-producing Escherichia coli conferred resistance to most β-lactams, except for carbapenems. To date, the transmission mechanism of bla, as the most common ESBLs subtype, in E. coli has received sustained attention around the worldwide, but the research on the pathogenicity of bla-bearing E.
View Article and Find Full Text PDFThe faecal microbiome of the Australian silver gull contains phylogenetically diverse ExPEC, aEPEC and Escherichia coli carrying the transmissible locus of stress tolerance.
Sci Total Environ
April 2024
Australian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, NSW, Australia. Electronic address:
Wildlife are implicated in the dissemination of antimicrobial resistance, but their roles as hosts for Escherichia coli that pose a threat to human and animal health is limited. Gulls (family Laridae) in particular, are known to carry diverse lineages of multiple-antibiotic resistant E. coli, including extra-intestinal pathogenic E.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!