We engineered a fluorescent fusion protein of lamin, by fusing the eleventh beta strand of GFP to the N-terminus of LMN-1 at the endogenous locus. When co-expressed with GFP1-10, GFP11::LMN-1 was observed at the nuclear periphery of a wide variety of somatic cells. Homozygous animals had normal numbers of viable embryos. However, the animals had a mild swimming defect. While not completely functional, the GFP11::LMN-1 strain is more healthy than other published fluorescent LMN-1 lines, making it a valuable reagent for studying lamins.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10751582 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.001022 | DOI Listing |
Publication Analysis
Top Keywords
split-gfp lamin
4
lamin tool
4
tool studying
4
studying lmn-1
4
lmn-1 dynamics
4
dynamics engineered
4
engineered fluorescent
4
fluorescent fusion
4
fusion protein
4
protein lamin
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!