AI Article Synopsis
- Zika virus infection can lead to serious health issues like Guillain-Barré syndrome and birth defects, prompting the need for effective vaccines for prevention.
- Researchers created a plant-based vaccine that effectively produced Zika virus proteins in Nicotiana benthamiana, showing promise in provoking immune responses in mice even at low doses.
- The vaccine not only enhanced immunity with an adjuvant but also allowed for the transfer of protective antibodies from immunized mother mice to their offspring, offering protection against Zika and Dengue viruses.
Article Abstract
Zika virus infection causes multiple clinical issues, including Guillain-Barré syndrome and neonatal malformation. Vaccination is considered as the only strategy for the prevention of ZIKV-induced clinical issues. This study developed a plant-based recombinant vaccine that transiently expressed the ZIKV envelope protein (ZikaEnv:aghFc) in Nicotiana benthamiana and evaluated the protective immunity afforded by it in immunocompetent mice. ZikaEnv:aghFc induced both humoral and cellular immunity at a low dose (1-5 μg). This immune-inducing potential was enhanced further when adjuvanted CIA09A. In addition, antigen-specific antibodies and neutralizing antibodies were vertically transferred from immunized females to their progeny and afforded both protective immunity to ZIKV and cross-protection to Dengue virus infection. These results suggest that our plant-based ZIKV vaccine provides a safe and efficient protective strategy with a competitive edge.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10752873 | PMC |
| http://dx.doi.org/10.1038/s41598-023-47428-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Imaging of viral replication in live cells by using split fluorescent protein-tagged reporter flaviviruses.
Virology
December 2024
The Centre for Infection and Immunity Studies, School of Medicine, Sun Yat-sen University, Shenzhen, Guangdong, 518107, China. Electronic address:
The knowledge on the life cycle of flaviviruses is still incomplete, and no direct-acting antivirals against their infections are clinically available. Herein, by screening via a Zika virus (ZIKV) replicon assay, we found that the N-terminus of NS2A exhibited great tolerance to the insertions of different split fluorescent proteins (split-FPs). Furthermore, both ZIKV and dengue virus encoding a split-FP-tagged NS2A propagated efficiently, and the split-FP-tagged ZIKVs had good genetic stability.
View Article and Find Full Text PDFRole of extracellular vesicles in the pathogenesis of mosquito-borne flaviviruses that impact public health.
J Biomed Sci
January 2025
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), 04510, Mexico City, Mexico.
Mosquito-borne flaviviruses represent a public health challenge due to the high-rate endemic infections, severe clinical outcomes, and the potential risk of emerging global outbreaks. Flavivirus disease pathogenesis converges on cellular factors from vectors and hosts, and their interactions are still unclear. Exosomes and microparticles are extracellular vesicles released from cells that mediate the intercellular communication necessary for maintaining homeostasis; however, they have been shown to be involved in disease establishment and progression.
View Article and Find Full Text PDFBuilding a growing genomic repository for maternal and fetal health through the PING Consortium.
Pediatr Res
January 2025
Center for Genetic Medicine, Children's National Research Institute, Washington, DC, USA.
Background: Prenatally transmitted viruses can cause severe damage to the developing brain. There is unexplained variability in prenatal brain injury and postnatal neurodevelopmental outcomes, suggesting disease modifiers. Of note, prenatal Zika infection can cause a spectrum of neurodevelopmental disorders, including congenital Zika syndrome.
View Article and Find Full Text PDFDengue and Zika virus NS4B proteins differ in topology and in determinants of ER membrane protein complex dependency.
J Virol
December 2024
1Division of Gastroenterology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Flaviviruses utilize the cellular endoplasmic reticulum (ER) for all aspects of their lifecycle. Genome replication and other viral activities take place in structures called replication organelles (ROs), which are invaginations induced in the ER membrane. Among the required elements for RO formation is the biogenesis of viral nonstructural proteins NS4A and NS4B.
View Article and Find Full Text PDFZika virus infections and associated risk factors among pregnant women in Gombe, Nigeria.
Virol Sin
December 2024
Department of Medical Laboratory Science, University of Maiduguri, College of Medical Sciences, P.M.B. 1069, Maiduguri, Nigeria. Electronic address:
Article Synopsis
- In-utero exposure to Zika virus (ZIKV) can result in severe outcomes like miscarriage and congenital Zika syndrome, prompting a study on the infection rates among pregnant women from February to April 2022.
- Researchers tested 200 pregnant women for ZIKV antibodies and found that 16.5% had ZIKV IgM, 10% had IgG, and 23% had neutralizing antibodies, indicating various stages of infection and immunity.
- The study also revealed that recent ZIKV infections were more common in sexually active women aged 20-29, with higher infection risks in first trimester and grand-multiparous women, although no significant link was found between ZIKV and specific pregnancy trimest
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!