Although near-infrared responsive photoelectrochemical (PEC) biosensors have less damage to biological components compared to UV-visible light, they still reveal an inferior response due to the rapid recombination of photogenerated electron-hole. In this study, a near-infrared-driven PEC biosensor is fabricated for microRNA (miRNA) detection via integrating photoelectricity and pyroelectricity. Upon the introduction of target miRNA-21, the exponential DNA amplifier is triggered based on enzyme-assisted strand displacement amplification (SDA), releasing multiple AgS reporter probes to hybridize with capture probes immobilized on a CdS-2-mercaptobenzimidazole (2MBI)-modified photoelectrode. As a result, under the stimulation of NIR, the photoelectric conversion of AgS NPs generates the photocurrents. In addition, due to the strong hole acceptor ability of MBI, the pyroelectric effect of CdS-2MBI nanocomposites is enhanced, which generates highly pyroelectro-induced charge separation efficiency and induces the pyroelectric current benefited from the spontaneous polarization of CdS-2MBI caused by the temperature variation under the function of AgS nanoheaters. Impressively, this PEC biosensor has achieved the sensitive and selective determination of miRNA-21 with a detection limit as low as 54 fM. Overall, this NIR-driven PEC biosensor based on pyroelectric and photoelectric effects opens up a new horizon for bioanalysis and early disease diagnosis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.analchem.3c04931 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A split-type near-infrared photoelectrochemical and colorimetric dual-mode biosensor for the high-performance determination of HepG2 cells.
Talanta
January 2025
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China. Electronic address:
Hepatocellular carcinoma (HCC) stands as a grave illness characterized by elevated death rates. Early identification plays a vital role in improving patient survival. Herein, a novel split-type dual-mode biosensor featuring with near-infrared photoelectronchemical (PEC) and colorimetric sensing characteristics was developed for the high-performance detection of HepG2 cells.
View Article and Find Full Text PDFNanoencapsulated Optical Fiber-Based PEC Microelectrode: Highly Sensitive and Specific Detection of NT-proBNP and Its Implantable Performance.
Anal Chem
January 2025
Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Microelectrodes offer exceptional sensitivity, rapid response, and versatility, making them ideal for real-time detection and monitoring applications. Photoelectrochemical (PEC) sensors have shown great value in many fields due to their high sensitivity, fast response, and ease of operation. Nevertheless, conventional PEC sensing relies on cumbersome external light sources and bulky electrodes, hindering its miniaturization and implantation, thereby limiting its application in real-time disease monitoring.
View Article and Find Full Text PDFBiS/BiO(OH) nanorods with internal electric field throughout the entire bulk phase as photoelectrochemical sensing platforms for CYFRA21-1 immunoassay.
Anal Chim Acta
February 2025
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, 250022, Jinan, PR China; Department of Chemistry, Sungkyunkwan University, 16419, Suwon, Republic of Korea. Electronic address:
Photoelectrochemical (PEC) immunosensors are highly promising tools for monitoring biochemical molecules. Constructing high-performance heterojunctions is a general method to improve the sensitivity of PEC immunosensors. The internal electric field (IEF) formed at the heterojunction interface plays a crucial role in coordinating the separation of photogenerated carriers.
View Article and Find Full Text PDFIn situ surface oxygen vacancy effect synergistic with internal polarization effect in BiFeO for photoelectrochemical detection of T4 DNA ligase.
Talanta
January 2025
Key Laboratory of Synthetic and Biological Colloids (Ministry of Education), School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China. Electronic address:
Recently, the field of cathode photoelectrochemistry has advanced significantly, yet there remained a dearth of innovative approaches in signal transmission strategies. This paper introduced a novel concept where the dopamine (DA)-engineered surface vacancy (Ov) effect on BiFeO microspheres synergistically interacted with the intrinsic polarization of the material, leading to a significantly enhanced photocurrent when compared to that of BiO or FeO alone without a built-in electric field. Based on this finding, we proposed a PEC biosensor that leveraged the competitive binding reaction between single-base nucleotides and DA for photocurrent output, wherein the T4 DNA ligase-mediated ligation reaction governed the production of single-base nucleotides.
View Article and Find Full Text PDFTarget-regulated AgS/FeOOH heterojunction activity: a direct label-free photoelectrochemical immunosensor.
Mikrochim Acta
January 2025
College of Geography and Environmental Sciences, College of Chemistry and Materials Science, Zhejiang Normal University, Jinhua, 321004, China.
Myoglobin (Mb), an important cardiac marker, plays a crucial role in diagnosing, monitoring, and evaluating the condition of patients with cardiovascular diseases. Here, we propose a label-free photoelectrochemical (PEC) sensor for the detection of Mb through target regulated the photoactivity of AgS/FeOOH heterojunction. The AgS/FeOOH nanospindles were synthesized and served as a sensing platform for the fabrication of bio-recognized process for Mb.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!