Pituitary gland function is regulated by the activity of various transcription factors that control cell fate decisions leading to cellular differentiation and hormone production. FOXO1 is necessary for normal somatotrope differentiation and function. Recent in vivo data implicate FOXO1 in the regulation of genes important for somatotrope differentiation including Gh1, Neurod4, and Pou1f1. In the current study, the somatotrope-like cell line GH3 was treated with a FOXO1 inhibitor, resulting in significant reduction in Neurod4 and Gh1 expression. Consistent with these findings, CRISPR/Cas9-mediated deletion of Foxo1 in GH3 cells significantly reduced expression of Gh1 and Neurod4. Chromatin immunoprecipitation sequencing identifies novel FOXO1 binding sites associated with the Neurod4, Gh1, and Pou1f1 genes. The FOXO1 binding site in the Neurod4 gene exhibits enhancer activity in somatotrope-like cells but not in gonadotrope-like cells. These data strongly suggest FOXO1 directly contributes to the transcriptional control of genes important for somatotrope differentiation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10922409 | PMC |
| http://dx.doi.org/10.1016/j.mce.2023.112128 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Article Synopsis
- This review examines the causes of headaches in patients with acromegaly and how various treatments for the condition affect headache symptoms.
- Headaches occur in 30-70% of acromegaly patients due to factors such as the tumor itself, hormonal changes, and other underlying issues, necessitating careful diagnosis and management.
- Despite treatments like surgery and medication normalizing hormone levels, headaches may still persist, highlighting the need for specialized headache management for these patients.
Long Noncoding RNAs Expressed in Mouse Pituitary Development and Mature Hormone-Producing Cells.
Endocrinology
October 2024
Department of Human Genetics, University of Michigan, Ann Arbor, MI 41809-5618, USA.
Mammalian genomes contain thousands of genes for long noncoding RNA (lncRNAs), some of which have been shown to affect protein coding gene expression through diverse mechanisms. The lncRNA transcripts are longer than 200 nucleotides and are often capped, spliced, and polyadenylated, but not translated into protein. Nuclear lncRNAs can modify chromatin structure and transcription in trans or cis by interacting with the DNA, forming R-loops, and recruiting regulatory proteins.
View Article and Find Full Text PDFParacrine FGF1 signaling directs pituitary architecture and size.
Proc Natl Acad Sci U S A
October 2024
Laboratoire de génétique moléculaire, Institut de recherches cliniques de Montréal, Montréal, QC H2W 1R7, Canada.
Organ architecture is established during development through intricate cell-cell communication mechanisms, yet the specific signals mediating these communications often remain elusive. Here, we used the anterior pituitary gland that harbors different interdigitated hormone-secreting homotypic cell networks to dissect cell-cell communication mechanisms operating during late development. We show that blocking differentiation of corticotrope cells leads to pituitary hypoplasia with a major effect on somatotrope cells that directly contact corticotropes.
View Article and Find Full Text PDFDynamic changes in insulin-like growth factor binding protein expression occur between embryonic and early post-hatch development in broiler chickens.
Poult Sci
November 2024
Department of Poultry Science, University of Georgia, Athens, GA 30602, USA. Electronic address:
Somatotropic gene expression has been altered by genetic selection, and developmental changes in insulin-like growth factor (IGF) and IGF binding protein (IGFBP) expression may contribute to rapid growth and muscle accretion in commercial broilers. The objective of this study was to evaluate changes in somatotropic axis activity between embryonic day (e) 12 and post-hatch day (d) 21. Liver and breast muscle (pectoralis major) were collected to measure gene expression, and blood was collected post-hatch to measure circulating IGFs.
View Article and Find Full Text PDFSex-differential testosterone response to long-term weight loss.
Int J Obes (Lond)
October 2024
Garvan institute of Medical Research, Darlinghurst, NSW, Australia.
Article Synopsis
- This study investigates how weight loss affects hormonal changes related to gonadal dysfunction in obese patients over three years, comparing different weight loss methods like diet and bariatric surgeries.
- Researchers measured various hormonal parameters in 61 adults with a BMI over 30 and found that weight loss significantly influenced testosterone levels, with men experiencing increases and women showing decreases.
- The results highlighted that women undergoing certain bariatric surgeries had notably lower testosterone levels compared to those on a diet, regardless of age or menopausal status, emphasizing the hormonal effects of different weight loss strategies.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!