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Unveiling the Probiotic Potential of the Anaerobic Bacterium sp. nov. C33 for Enhancing Nile Tilapia () Cultures. | LitMetric

AI Article Synopsis

Article Abstract

The bacterium strain sp. C33 was isolated from the intestinal microbial content of Nile tilapia (. ) under anaerobic conditions. Given that species are recognized as primary constituents of the intestinal microbiota in cultured Nile tilapia by culture-independent techniques, the adaptability of the C33 strain to the host gastrointestinal conditions, its antibacterial activity against aquaculture bacterial and its antibiotic susceptibility were assessed. The genome of C33 was sequenced, assembled, annotated, and subjected to functional inference, particularly regarding pinpointed probiotic activities. Furthermore, phylogenomic comparative analyses were performed including closely reported strains/species relatives. Comparative genomics with closely related species disclosed that the isolate is not phylogenetically identical to other species, displaying an approximately 5% sequence divergence from . and a 13% sequence divergence from . It can be distinguished from other species through physiological and biochemical criteria. Whole-genome annotation highlighted that sp. nov. C33 possesses a set of genes that may contribute to antagonism against competing bacteria and has specific symbiotic adaptations in fish. Additional in vivo experiments should be carried out to verify favorable features, reinforcing its potential as a probiotic bacterium.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10745334PMC
http://dx.doi.org/10.3390/microorganisms11122922DOI Listing

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