Interruption of Lymph Flow Worsens the Skin Inflammation Caused by Saprophytic .

Lymphedema is often complicated by chronic inflammation, leading to fibrosis, fat deposition, and inhibition of lymphangiogenesis. This study aimed to verify whether lymphedema itself or together with commensal bacterial flora infection contributes to the severity of local inflammation. Edema was induced by interruption of the lymph flow in the rat's hind limb. Immune cell infiltrates were examined by flow cytometry and immunohistochemistry. Nine-day edema alone did not affect immune cell content in the skin but resulted in a decrease in CD4 T helper lymphocytes and monocytes in the draining popliteal lymph nodes. In turn, local saprophytic infection of the edematous limb resulted in dense infiltrates of CD68 macrophages and monocytes, MHC class II antigen-presenting cells, CD90 stem cells, thymocytes, and immature B cells in the skin, accompanied by a simultaneous reduction in density of CD4 T helper lymphocytes and monocytes, OX62 dendritic cells, CD68 macrophages and monocytes, HiS48 granulocytes, CD90 stem cells, thymocytes, and immature B cells in the draining popliteal lymph nodes. These results indicate that the combination of edema and saprophytic bacteria infection induces severe inflammation in the peripheral tissues and results in a delay of antibacterial protection processes in neighboring lymphatic organs.