Characterization of Strains Isolated from Various Poultry Species in Poland.

Antibiotics (Basel)

Department of Veterinary Prevention and Avian Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-033 Lublin, Poland.

Published: November 2023

() is one of the common pathogens found in poultry flocks, resulting in serious economic losses for the poultry industry due to high mortality, reduced growth rate, poor feed conversion, increased condemnations, and high treatment costs. The aim of this study was to phenotypically characterize phylogenetic relationships and assess the presence of resistance gene strains of obtained from various poultry species in Poland. A total of 57 isolates of were included in this study. A polymerase chain reaction (PCR) and matrix assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) were used for identification of the strains. The phylogenetic relationship of the isolates was determined by analysing the B gene sequence. The susceptibility to antibiotics was evaluated by minimum inhibitory concentration (MIC) in liquid media. All of the field strains of were grouped into one of two clades resulting from B gene sequencing. High MIC and MIC values were obtained for gentamycin, amikacin, and colistin. Low MIC and MIC values were obtained for amoxicillin cefuroxime, cefoperazone, piperacillin, and trimethoprim/sulfamethoxazole. Among the resistance genes, and were identified most frequently. This is the first phenotypic characterization of strains obtained from poultry flocks in Poland.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10740677PMC
http://dx.doi.org/10.3390/antibiotics12121648DOI Listing

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