Polysaccharides, the major active ingredient and quality control indicator of Polygomatum cyrtonema are in need of elucidation for its in vitro fermentation characteristics. This study aimed to investigate the structural characteristics of the homogeneous Polygomatum cyrtonema polysaccharide (PCP-80 %) and its effects on human intestinal bacteria and short chain fatty acids (SCFAs) production during the in vitro fermentation. The results revealed that PCP-80 % was yielded in 10.44 % and the molecular weight was identified to be 4.1 kDa. PCP-80 % exhibited a smooth, porous, irregular sheet structure and provided good thermal stability. The analysis of Gas chromatograph-mass spectrometer (GC-MS) suggested that PCP-80 % contained six glycosidic bonds, with 2,1-linked-Fruf residues accounted for a largest proportion. Nuclear magnetic resonance (NMR) provided additional evidence that the partial structure of PCP-80 % probably consists of →1)-β-D-Fruf-(2 → as the main chain, accompanied by side chains dominated by →6)-β-D-Fruf-(2→. Besides, PCP-80 % promoted the production of SCFAs and increased the relative abundance of beneficial bacteria such as Megamonas, Bifidobacterium and Phascolarctobacterium during in vitro colonic fermentation, which changed the composition of the intestinal microbiota. These findings indicated that Polygomatum cyrtonema polysaccharides were able to modulate the structure and composition of the intestinal bacteria flora and had potential probiotic properties.
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http://dx.doi.org/10.1016/j.ijbiomac.2023.128877 | DOI Listing |
Curr Microbiol
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School of Chinese Materia Medica, Beijing University of Chinese Medicine, Yangguang South Street, Fangshan, Beijing, 102488, China.
Astragalus polysaccharides (APS) are known for their prebiotic properties, and fermentation by probiotics is a promising strategy to enhance the prebiotic activity of polysaccharides. In this study, Lactobacillus rhamnosus was used to ferment APS, and response surface methodology was applied to optimize the fermentation parameters. The optimal conditions were determined as follows: 10.
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Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar, Odisha 761003, India. Electronic address:
Ophthalmic disorders significantly impact global health, affecting millions worldwide. Conventional treatments often face challenges related to poor bioavailability and short residence times on the ocular surface. In recent years, in-situ gels prepared using different natural gums including gellan gum has been investigated as a viable means of improving ocular medication delivery.
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November 2024
Poznań University of Life Sciences, Department of Animal Nutrition, Wołyńska 33, 60-637 Poznań, Poland. Electronic address:
Greenhouse gas (GHG) emissions from livestock ruminants, particularly methane (CH), nitrous oxide, and indirectly ammonia (NH) significantly contribute to climate change and global warming. Conventional monoculture swards for cattle feeding, such as perennial ryegrass or Italian ryegrass, usually require substantial fertiliser inputs. Such management elevates soil mineral nitrogen levels, resulting in GHG emissions and potential water contamination.
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Qilu University of Technology (Shandong Academy of Sciences), Shandong Food Ferment Industry Research & Design Institute, Jinan 250000, China. Electronic address:
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December 2024
Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Jiaxing Institute of Future Food, Jiaxing 314000, China; Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address:
The nutritional properties, anti-nutritional factors, and in vitro digestion characteristics of chickpeas after solid-state fermentation (SSF) with autochthonous microorganisms were investigated. Two strains (P.pentosaceus LFSBB12 & P.
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