Identification of G Protein-Coupled Receptors (GPCRs) Associated with -Cyhalothrin Detoxification in .

While previous studies have reported G protein-coupled receptor (GPCR)-mediated insecticide resistance in various arthropods, the understanding of GPCR-associated resistance mechanisms in remains limited. In this study, a total of 95 genes categorized into four families were identified in . Results revealed high expression levels of the majority of the during the first larval stage and in the head of Exposure to -cyhalothrin significantly increased the expression of 15 , including , which is highly expressed in all larval stages and shows the highest expression in the midgut. RNA interference (RNAi) demonstrated that downregulation of leads to reduced expression of key resistance-related genes and a decreased tolerance of larvae to -cyhalothrin. These findings indicate that plays a crucial role in regulating the expression of detoxifying genes involved in -cyhalothrin resistance, offering valuable insights for the development of more effective pest control strategies.