Ca plays a crucial role in cell signaling, cytosolic Ca can change up to 10,000-fold in concentration due to the action of Ca-ATPases, including PMCA, SERCA and SCR. The regulation and balance of these enzymes are essential to maintain cytosolic Ca homeostasis. Our laboratory has discovered a novel PMCA regulatory system, involving acetylated tubulin alone or in combination with membrane lipids. This regulation controls cytosolic Ca levels and influences cellular properties such as erythrocyte rheology. This review summarizes the findings on the regulatory mechanism of PMCA activity by acetylated tubulin in combination with lipids. The combination of tubulin cytoskeleton and membrane lipids suggests a novel regulatory system for PMCA, which consequently affects cytosolic Ca content, depending on cytoskeletal and plasma membrane dynamics. Understanding the interaction between acetylated tubulin, lipids and PMCA activity provides new insights into Ca signaling and cell function. Further research may shed light on potential therapeutic targets for diseases related to Ca dysregulation. This discovery contributes to a broader understanding of cellular processes and offers opportunities to develop innovative approaches to treat Ca-related disorders. By elucidating the complex regulatory mechanisms of Ca homeostasis, we advance our understanding of cell biology and its implications for human health.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12013-023-01206-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Stress causes lipid droplet accumulation in chondrocytes by impairing microtubules.
Osteoarthritis Cartilage
December 2024
Department of Oral Anatomy and Physiology and TMD, College of Stomatology, the Fourth Military Medical University. Xi'an, China; Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Shanghai Stomatological Hospital, Fudan University, Shanghai, China. Electronic address:
Objective: Abnormal mechanical stress is intimately coupled with osteoarthritis (OA). Microtubules play a vital role in the regulation of mechanotransduction and intracellular transport. The purpose of the present study was to investigate the impact of stress-induced microtubule impairment on intracellular transport and lipid droplet (LD) accumulation in chondrocytes.
View Article and Find Full Text PDFTubulin-Based Microtentacles Aid in Heterotypic Clustering of Neutrophil-Differentiated HL-60 Cells and Breast Tumor Cells.
Adv Sci (Weinh)
December 2024
Marlene and Stewart Greenebaum NCI Comprehensive Cancer Center, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD, 21201, USA.
Circulating tumor cells (CTCs) travel through the vasculature to seed secondary sites and serve as direct precursors of metastatic outgrowth for many solid tumors. Heterotypic cell clusters form between CTCs and white blood cells (WBCs) and recent studies report that a majority of these WBCs are neutrophils in patient and mouse models. The lab discovered that CTCs produce tubulin-based protrusions, microtentacles (McTNs), which promote reattachment, retention in distant sites during metastasis and formation of tumor cell clusters.
View Article and Find Full Text PDFInvestigating the Role of Primary Cilia and Bone Morphogenetic Protein Signaling in Periodontal Ligament Response to Orthodontic Strain In Vivo and In Vitro: A Pilot Study.
Int J Mol Sci
November 2024
Department of Orthodontics, University of Bonn, 53111 Bonn, Germany.
Periodontal ligament (PDL) cells are crucial for mechanosensation and mechanotransduction within the PDL, yet the role of primary cilia in orthodontic force transmission has not been examined. While bone morphogenetic protein (BMP) signaling significantly influences ciliary function, its effect on cellular responses to mechanical stress has not been investigated. This study aims to investigate whether primary cilia and BMP signaling are involved in the periodontal ligament's response to orthodontic tooth movement and the resultant mechanical strain.
View Article and Find Full Text PDFSIRT2 Inhibition by AGK2 Promotes Perinuclear Cytoskeletal Organisation and Reduces Invasiveness of MDA-MB-231 Triple-Negative Breast Cancer Cells in Confined In Vitro Models.
Cells
December 2024
Department of Biosciences, Durham University, Durham DH1 3LE, UK.
Triple-negative breast cancer (TNBC) is a highly aggressive breast cancer subtype characterised by the absence of targetable hormone receptors and increased metastatic rates. As nuclear softening strongly contributes to TNBC's enhanced metastatic capacity, increasing the nuclear stiffness of TNBC cells may present a promising therapeutic avenue. Previous evidence has demonstrated the ability of Sirtuin 2 (SIRT2) inhibition to induce cytoskeletal reorganisation, a key factor in regulating nuclear mechanics.
View Article and Find Full Text PDFArticle Synopsis
- Exfoliation Syndrome (XFS) is an age-related condition affecting the eye, leading to the accumulation of fibrillar materials that can cause Exfoliation Glaucoma (XFG), a form of secondary open-angle glaucoma.
- XFG is linked to neurodegenerative disease features and characterized by mitochondrial dysfunction, including impaired energy production and increased reactive oxygen species (ROS) in affected cells.
- Research shows that treating XFG patient-derived cells with agents like Urolithin A and Nicotinamide Ribose can help improve mitochondrial function and reduce ROS, suggesting a potential therapeutic approach for XFG.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!