Cytosporones with Anti-Inflammatory Activities from the Mangrove Endophytic Fungus sp. QYM-13.

Six previously undescribed cytosporone derivatives (phomotones A-E (-) and phomotone F ()), two new spiro-alkanol phombistenes A-B (-), and seven known analogs (-) were isolated from the mangrove endophytic fungus sp. QYM-13. The structures of these compounds were elucidated using spectroscopic data analysis, electronic circular dichroism (ECD), and C NMR calculations. Compound features an unprecedented 1,6-dioxaspiro[4.5]decane ring system. All isolates were evaluated for their inhibitory effect on nitric oxide (NO) in LPS-induced RAW264.7 cells. The results showed that compounds , , and exhibited potent bioactivities by comparing with positive control. Then, compound displayed the anti-inflammatory effect by inhibiting the MAPK/NF-B signaling pathways. Molecular docking further revealed the possible mechanism of compound interaction with ERK protein.