The development of abamectin resistance in Liriomyza trifolii and its contribution to thermotolerance.

Background: Liriomyza trifolii is an economically significant, invasive pest of horticultural and vegetable crops. The larvae form tunnels in foliage and hasten senescence and death. Outbreaks of L. trifolii often erupt in hot weather and are driven by thermotolerance; furthermore, the poor effectiveness of pesticides has made outbreaks more severe. But it is still unclear whether the development of insecticide tolerance will contribute to thermotolerance in L. trifolii.

Results: To explore potential synergistic relationships between insecticide exposure and thermotolerance in L. trifolii, we first generated an abamectin-resistant (AB-R) strain. Knockdown behavior, eclosion and survival rates, and expression levels of genes encoding heat shock proteins (Hsps) in L. trifolii were then examined in AB-R and abamectin-susceptible (AB-S) strains. Our results demonstrated that long-term selection pressure for abamectin resistance made L. trifolii more prone to develop cross-resistance to other insecticides containing similar ingredients. Furthermore, the AB-R strain exhibited enhanced thermotolerance and possessed an elevated critical thermal maximum temperature, and upregulated expression levels of Hsps during heat stress.

Conclusion: Collectively, our results indicate that thermal adaptation in L. trifolii was accompanied by emerging abamectin resistance. This study provides a theoretical basis for investigating the synergistic or cross-adaptive mechanisms that insects use to cope with adversity and demonstrates the complexity of insect adaptation to environmental and chemical stress. © 2023 Society of Chemical Industry.