AI Article Synopsis
- - The study investigates the role of Triosephosphate isomerase 1 (TPI1), a key glycolytic enzyme, in lung adenocarcinoma (LUAD), highlighting its significant expression in tumors and link to glucose metabolism.
- - Bioinformatic analysis revealed that high levels of TPI1 are associated with metastasis and poor prognosis in LUAD, supported by experimental methods such as Western blot and RT-qPCR.
- - Results indicate that TPI1 enhances metastasis by promoting epithelial-mesenchymal transition (EMT) in LUAD cells, operating through the MAPK/ERK signaling pathway.
Article Abstract
Background: Triosephosphate isomerase 1 (TPI1), as a widely involved glycolytic enzyme, plays a significant role in glucose metabolism and is highly expressed in various tumors. However, its role in lung adenocarcinoma (LUAD) remains incompletely understood.
Methods: Through bioinformatic analysis, we identified a positive association between high expression of TPI1 and metastasis in LUAD. Western blot, RT-qPCR, wound healing assays and transwell experiments, were employed to investigate potential mechanisms.
Results: In this study, bioinformatic analysis showed that high expression of TPI1 was associated with poor prognosis in LUAD patients. We examined the expression of TPI1 in 29 paired LUAD tissues and found that TPI1 expression was higher in LUAD tissues than in paired adjacent noncancerous tissues. Meanwhile, overexpression of TPI1 promoted the epithelial-mesenchymal transition (EMT) process in LUAD cells, while silencing TPI1 weakened the EMT process. Furthermore, TPI1 was shown to regulate EMT through the MAPK/ERK signaling pathway.
Conclusion: TPI1 promotes LUAD metastasis by activating the MAPK/ERK signaling pathway.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10834191 | PMC |
| http://dx.doi.org/10.1111/1759-7714.15196 | DOI Listing |
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