AI Article Synopsis

  • The organization and function of cellular membranes depend on various factors, including specific amino acid motifs that expand protein functions.
  • Researchers identified a unique motif in chordata EHBP1 proteins that is essential for their interaction with syndapin I, a protein important in shaping membranes in neuron cells.
  • Studies in rat hippocampal neurons revealed that EHBP1 plays a crucial role in dendritic development, with its function depending on its interaction with syndapin I and involving additional domains, highlighting the importance of actin nucleation for proper neuronal growth.

Article Abstract

The coordinated action of a plethora of factors is required for the organization and dynamics of membranous structures critically underlying the development and function of cells, organs, and organisms. The evolutionary acquisition of additional amino acid motifs allows for expansion and/or specification of protein functions. We identify a thus far unrecognized motif specific for chordata EHBP1 proteins and demonstrate that this motif is critically required for interaction with syndapin I, an F-BAR domain-containing, membrane-shaping protein predominantly expressed in neurons. Gain-of-function and loss-of-function studies in rat primary hippocampal neurons (of mixed sexes) unraveled that EHBP1 has an important role in neuromorphogenesis. Surprisingly, our analyses uncovered that this newly identified function of EHBP1 did not require the domain responsible for Rab GTPase binding but was strictly dependent on EHBP1's syndapin I binding interface and on the presence of syndapin I in the developing neurons. These findings were underscored by temporally and spatially remarkable overlapping dynamics of EHBP1 and syndapin I at nascent dendritic branch sites. In addition, rescue experiments demonstrated the necessity of two additional EHBP1 domains for dendritic arborization, the C2 and CH domains. Importantly, the additionally uncovered critical involvement of the actin nucleator Cobl in EHBP1 functions suggested that not only static association with F-actin via EHBP1's CH domain is important for dendritic arbor formation but also actin nucleation. Syndapin interactions organize ternary protein complexes composed of EHBP1, syndapin I, and Cobl, and our functional data show that only together these factors give rise to proper cell shape during neuronal development.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10860635PMC
http://dx.doi.org/10.1523/JNEUROSCI.0236-23.2023DOI Listing

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