Nitrous oxide emissions are driven by environmental conditions rather than nitrogen application methods in a perennial hayfield.

Agricultural best management practices (BMPs) intended to solve one environmental challenge may have unintended climate impacts. For example, manure injection is often promoted for its potential to reduce runoff and nitrogen (N) loss as NH , but the practice has been shown to increase N O, a powerful greenhouse gas, compared to surface application. Urease inhibitor application with N fertilizer is another BMP that can enhance N retention by reducing NH emissions, but its impact on N O emissions is mixed. Thus, we measured N O, CO , soil mineral N availability, soil moisture, soil temperature, and yield in a 2-year perennial hayfield trial with four fertilization treatments (manure injection, manure broadcast, synthetic urea, and control) applied with or without a urease inhibitor in Alburgh, VT. We used linear models to examine treatment effects on daily and cumulative N O emissions and a boosted regression tree (BRT) model to identify the most important drivers of daily N O fluxes in our trial. While fertilization type had a significant impact on N O fluxes (p < 0.05), our treatments explained an unexpectedly small amount of the variation in emissions (R  = 0.042), and urease inhibitor had no effect. Instead, soil moisture was the most important predictor of daily N O fluxes (39.7% relative influence in BRT model), followed by CO fluxes, soil inorganic N, and soil temperature. Soil moisture and temperature interacted to produce the largest daily N O fluxes when both were relatively high, suggesting that injecting manure during dry periods or during wet but cool periods could reduce its climate impacts.