FRET-based sensors are utilized for real-time measurements of cellular tension. However, transfection of the sensor gene shows low efficacy and is only effective for a short period. Reporter mice expressing such sensors have been developed, but sensor fluorescence has not been measured successfully using conventional confocal microscopy. Therefore, methods for spatiotemporal measurement of cellular tension in vivo or ex vivo are still limited. We established a reporter mouse line expressing FRET-based actinin tension sensors consisting of EGFP as the donor and mCherry as the acceptor and whose FRET ratio change is observable with confocal microscopy. Tension-induced changes in FRET signals were monitored in the aorta and tail tendon fascicles, as well as aortic smooth muscle cells isolated from these mice. The pattern of FRET changes was distinctive, depending on tissue type. Indeed, aortic smooth muscle cells exhibit different sensitivity to macroscopic tensile strain in situ and in an isolated state. This mouse strain will enable novel types of biomechanical investigations of cell functions in important physiological events.
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http://dx.doi.org/10.1038/s41598-023-50142-z | DOI Listing |
Int J Biol Macromol
January 2025
Neurosurgery Department, the First Affiliated Hospital of Ningbo University, Ningbo 315000, China. Electronic address:
Despite the worldwide prevalence of Parkinson's disease (PD), there are currently no effective methods for treating or preventing α-synucleinopathy. Research has demonstrated that small molecules are capable of preventing α-synuclein aggregation and the associated neurotoxicity. Nonetheless, the specific anti-amyloid mechanism of these compounds is not thoroughly comprehended in detail.
View Article and Find Full Text PDFBiophys J
January 2025
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; Center for Physics and Chemistry of Living Systems, Tel Aviv University, Tel Aviv, Israel. Electronic address:
Migrasomes, the vesicle-like membrane micro-structures, arise on the retraction fibers (RFs), the branched nano-tubules pulled out of cell plasma membranes during cell migration and shaped by membrane tension. Migrasomes form in two steps: a local RF bulging is followed by a protein-dependent stabilization of the emerging spherical bulge. Here we addressed theoretically and experimentally the previously unexplored mechanism of bulging of membrane tubular systems.
View Article and Find Full Text PDFBackground: Yes-associated protein (YAP) is a crucial mechanosensor involved in mechanotransduction, but its role in regulating mechanical force-induced bone remodeling during orthodontic tooth movement (OTM) is unclear. This study aims to elucidate the relationship between mechanotransduction and mechanical force-induced alveolar bone remodeling during OTM.
Results: Our study confirms an asynchronous (temporal and spatial sequence) remodeling pattern of the alveolar bone under mechanical force during OTM.
J Orofac Orthop
January 2025
Department of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Topkapı, Gureba Hastanesi Cd, No. 69, 34093, Istanbul, Turkey.
Purpose: The aim of this study was to examine the effect of methylphenidate, prescribed for individuals with attention deficit hyperactivity disorder (ADHD), on orthodontic tooth movement (OTM) and root resorption.
Methods: In all, 30 rats were divided into (1) control (C), (2) constant (MCD), and (3) increasing dose of methylphenidate (MID) groups and 2 subgroups for each of them (nonorthodontic (30 days)/orthodontic (44 days)). After receiving saline or methylphenidate for 30 days, rats in the nonorthodontic groups were euthanized (n = 5/group).
Nat Commun
January 2025
Center for Physics and Chemistry of Living Systems, Tel Aviv University, Tel Aviv, Israel.
Propagation of membrane tension mediates mechanical signal transduction along surfaces of live cells and sets the time scale of mechanical equilibration of cell membranes. Recent studies in several cell types and under different conditions revealed a strikingly wide variation range of the tension propagation speeds including extremely low ones. The latter suggests a possibility of long-living inhomogeneities of membrane tension crucially affecting mechano-sensitive membrane processes.
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